神经生长因子受体重组兔单抗

商品属性

产品介绍
英文名称NGFR/p75NTR Recombinant Rabbit mAb

中文名称神经生长因子受体重组兔单抗

别    名TNR16_HUMAN; Tumor necrosis factor receptor superfamily member 16; Gp80-LNGFR; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor(NGF receptor 1 Publication); p75 ICD; CD271; TNFRSF16; p75NTR; p75(NTR); Gp80-LNGFR; p75 NGF Receptor;  

研究领域细胞生物  神经生物学  信号转导  

抗体来源Rabbit

克隆类型Recombinant

克 隆 号5D4

交叉反应Human,Mouse,Rat

产品应用IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:50-200

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量44 kDa

检测分子量

细胞定位细胞核 细胞浆 细胞膜 细胞外基质

性    状Liquid

浓    度1mg/ml

免 疫 原A synthesized peptide derived from human p75 NTR: 350-400

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells. NGF is important for the development, differentiation and survival of a variety of neuronal and non-neuronal cells. Its action is mediated by binding to two distinct receptors, the high affinity p140 and low affinity p75. p75NGFR binds neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, and NT-6. p75NGFR belongs to the TNF-R superfamily and is reported to mediate NGF-induced apoptosis.

靶点与功能

GluN1（GRIN1，NMDA受体亚基1）是离子型谷氨酸受体NMDA受体的必需亚基，通过与GluN2（A-D）亚基形成异四聚体通道，介导Ca²⁺内流和突触可塑性：

突触传递：作为NMDA受体的“骨架亚基”，GluN1负责配体结合（甘氨酸结合位点）和离子通道孔道形成，在长时程增强（LTP） 和学习记忆中起核心作用；

神经疾病机制：其表达异常或功能失调与阿尔茨海默病（Aβ抑制GluN1活性）、癫痫（GluN1过度激活导致 excitotoxicity） 及抑郁症（NMDA受体拮抗剂快速起效）密切相关；

亚细胞定位：主要分布于大脑皮层、海马CA1区及脊髓神经元突触后膜，免疫组化中呈典型的“神经元膜/突触”阳性信号。

实验操作关键要点

WB检测（海马神经元样本）：

样本处理：原代海马神经元（DIV14-21）用含蛋白酶抑制剂（1mM PMSF） 和磷酸酶抑制剂（PhosSTOP） 的RIPA裂解液冰上裂解30分钟，12000g离心取上清；

电泳条件：7.5% SDS-PAGE凝胶（因分子量较大），恒压80V浓缩胶、120V分离胶，转膜使用0.45μm PVDF膜（300mA恒流2小时）；

抗体孵育：一抗1:1000稀释（5% BSA+TBST），4℃孵育过夜，HRP二抗（山羊抗兔）1:5000室温孵育1小时，曝光时间60秒（103kDa处可见清晰条带）。

IHC-P脑组织染色：

抗原修复：柠檬酸盐缓冲液（pH6.0）微波修复20分钟（石蜡切片），神经元膜信号显著增强；

阳性定位：海马CA1区锥体细胞胞膜/树突棘呈棕黄色强阳性，皮层神经元弱阳性，胶质细胞阴性（特异性标志）；

对照设置：GRIN1 KO小鼠脑组织（阴性对照）和野生型小鼠海马组织（阳性对照）。

IF突触共定位实验：

与PSD95抗体共染（突触后致密区标志物），GluN1（绿色）与PSD95（红色）在神经元树突棘共定位，激光共聚焦可观察“珍珠链”样突触分布。

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