跨膜受体蛋白Notch-1重组兔单抗

商品属性

产品介绍
英文名称Notch1 Recombinant Rabbit mAb

中文名称跨膜受体蛋白Notch-1重组兔单抗

别    名Neurogenic locus notch homolog protein 1; Notch 1 extracellular truncation; Notch 1 intracellular domain; hN1; Lin-12; LIN12; MIS6; Motch A; mT14; Neurogenic locus notch homolog protein 1; Neurogenic locus notch protein homolog; NICD; Notch1 intracellular domain; NOTC1_HUMAN; Notch 1; NOTCH; Notch gene homolog 1(Drosophila); Notch homolog 1 translocation associated(Drosophila); Notch homolog 1, translocation-associated(Drosophila); NOTCH, Drosophila, homolog of, 1; p300; TAN 1; TAN1; Translocation Associated Notch Homolog; Translocation Associated Notch Homolog; Translocation associated notch protein TAN 1; Translocation-associated notch protein TAN-1.  

抗体来源Rabbit

克隆类型Recombinant

克 隆 号16B1

交叉反应Human,Mouse,Rat

产品应用WB=1:500-2000,IHC-P=1:200-800,IHC-F=1:200-800,IF=1:200-800,Flow-Cyt=1:50-100,ICC/IF=1:50-200

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量271 kDa

检测分子量

细胞定位细胞核 细胞膜

性    状Liquid

浓    度1mg/ml

免 疫 原A synthesized peptide derived from human Notch 1: 2500-2555/2555

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. [provided by RefSeq, Jul 2008].

靶点与功能

神经元特异性烯醇化酶（NSE，γ烯醇化酶）是糖酵解途径的关键酶（γγ型同源二聚体，分子量约90kDa），核心功能与临床价值包括：

组织特异性：主要表达于神经元、神经内分泌细胞及相关肿瘤（如小细胞肺癌、神经母细胞瘤），正常血清参考值≤16.3ng/mL；

肿瘤诊断价值：小细胞肺癌患者血清NSE水平显著升高（阳性率70%-80%），与肿瘤分期、转移及预后密切相关（NSE>35ng/mL提示生存期缩短）；

神经保护作用：在中枢神经系统损伤（如脑缺血、创伤）后，NSE从受损神经元释放，可作为神经损伤程度的评估指标。

实验操作关键要点

IHC-P肿瘤组织染色（小细胞肺癌样本）：

抗原修复：柠檬酸盐缓冲液（pH6.0）微波修复15分钟（石蜡切片），胞质信号显著增强；

阳性定位：肿瘤细胞胞质呈棕黄色颗粒状阳性，小细胞肺癌组织阳性率＞90%，腺癌/鳞癌阴性（可与TTF-1联合检测提高特异性）；

对照设置：正常脑组织（神经元胞质阳性对照）和肺腺癌组织（阴性对照）。

WB验证（神经母细胞瘤细胞系）：

样本制备：SK-N-SH细胞用含蛋白酶抑制剂（1mM PMSF） 的RIPA裂解液冰上裂解30分钟，12000g离心取上清；

电泳条件：10% SDS-PAGE凝胶，恒压80V浓缩胶、120V分离胶，转膜使用0.45μm PVDF膜（250mA恒流1.5小时）；

抗体孵育：一抗1:1000稀释（5% BSA+TBST），4℃孵育过夜，HRP二抗（山羊抗兔）1:5000室温孵育1小时，曝光时间45秒（90kDa处可见清晰二聚体条带）。

ELISA血清定量检测：

包被优化：抗体用碳酸盐缓冲液（pH9.6）稀释至2μg/mL，4℃包被过夜；

样本处理：血清需避免溶血（红细胞含NSE会干扰结果），1:10稀释后37℃孵育1.5小时；

显色控制：TMB底物显色10分钟，2M H₂SO₄终止，450nm读取OD值（标准曲线范围0.1-100ng/mL）。

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