商品属性

产品介绍Neuronal Marker

Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.

商品详情

英文名称TUBB3 (Neuronal Marker) Mouse mAb

中文名称微管蛋白β3（神经标志物）单克隆抗体

别    名Tubulin beta 3; beta III Tubulin; Tubb3; Tubulin beta-3; beta 4; MC1R; TUBB 3; TUBB 4; TUBB3; TUBB4; Tubulin beta 3 chain; Tubulin beta 4; Tubulin beta III; Tubulin beta-3 chain; Neuron-specific class III beta-tubulin; Syntaxin III; Neuron specific beta III Tubulin; Tubulin beta-4 chain; Tubulin beta-III; beta-4; CDCBM; CFEOM3A; M(beta)3; M(beta)6; Neuron-specific class III beta-tubulin; QccE-11995; QccE-15186; TBB3_HUMAN; Tubulin beta 4; Tubulin beta-4 chain.  TUJ1

研究领域细胞生物  神经生物学  细胞类型标志物  细胞骨架  

抗体来源Mouse

克隆类型Monoclonal

克 隆 号6F12

交叉反应Human,Mouse,Rat

产品应用WB=1:1000-10000,IHC-P=1:200-1000,IHC-F=1:200-1000,IF=1:200-1000,Flow-Cyt=1μg/Test

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量50 kDa

检测分子量

细胞定位细胞浆

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human TUBB3

亚    型IgG

纯化方法affinity purified by Protein G

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

靶点与功能

TUBB3是微管蛋白β家族成员，主要表达于中枢和外周神经系统的神经元，参与神经元突起生长、轴突运输和突触形成等关键过程。在神经母细胞瘤、小细胞肺癌、神经内分泌肿瘤中高表达，可作为神经源性肿瘤诊断标志物；同时在阿尔茨海默病、帕金森病等神经退行性疾病中，TUBB3的异常磷酸化与微管稳定性下降密切相关，是神经损伤修复研究的重要靶点。

实验操作关键要点

WB优化：

样本处理：脑组织或神经细胞需用含1mM PMSF的RIPA裂解液（添加10mM NaF抑制磷酸酶），冰上裂解30分钟；

电泳条件：10% SDS-PAGE凝胶，恒压80V浓缩胶、120V分离胶，确保50kDa条带与内参（如β-actin）清晰分离；

抗体孵育：一抗推荐1:2000稀释（5% BSA+TBST），4℃摇床孵育过夜，HRP标记二抗（1:5000）室温孵育1小时。

IF双标染色技巧：

与GFAP（星形胶质细胞标志物）共染时，建议TUBB3一抗用Cy3标记（红色荧光），GFAP用FITC标记（绿色荧光），DAPI复染细胞核（蓝色）；

透化条件：0.3% Triton X-100室温透化15分钟，可增强神经元突起信号；

封闭液：5%驴血清+0.1% BSA，室温封闭1小时，减少非特异性结合。

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