程序性死亡1（CD279）单克隆抗体

商品属性

产品介绍
英文名称PD-1 Mouse mAb

中文名称程序性死亡1（CD279）单克隆抗体

别    名Programmed cell death protein 1; CD279; CD279 antigen; hPD 1; hPD-1; hSLE1; PD 1; PD1; PDCD 1; PDCD1; PDCD1_HUMAN; Programmed cell death 1; Protein PD 1; Protein PD-1; SLEB2; Systemic lupus erythematosus susceptibility 2.  

研究领域肿瘤  细胞生物  免疫学  

抗体来源Mouse

克隆类型Monoclonal

克 隆 号1C4

交叉反应Human

产品应用IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量32 kDa

检测分子量

细胞定位细胞膜

性    状Liquid

浓    度1mg/1ml

免 疫 原Recombinant human PD1 <Extracellular>

亚    型IgG1

纯化方法affinity purified by Protein G

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.

CD279 is an immunoglobulin superfamily member, also known as Programmed Cell Death 1. Programmed Cell Death 1 is expressed on a subset of CD4-CD8 thymocytes, and on activated T and B cells. Programmed Cell Death 1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The Programmed Cell Death 1 ligands, PDL1 (also known as B7H1) and PDL2 (B7DC), are members of the B7 immunoglobulin superfamily.

靶点与功能

PDGFRβ（Platelet-Derived Growth Factor Receptor Beta）是一种跨膜酪氨酸激酶受体，与配体PDGF-BB结合后发生二聚化激活，通过磷酸化下游分子（如PI3K、PLCγ）调控：

血管平滑肌细胞增殖：参与血管壁修复及新生血管成熟，与动脉粥样硬化斑块稳定性相关；

间质纤维化：激活肝星形细胞（HSC）转化为肌成纤维细胞，促进细胞外基质（ECM）沉积，是肝纤维化、肾间质纤维化的核心驱动因子；

肿瘤微环境：肿瘤相关成纤维细胞（CAF）高表达PDGFRβ，通过分泌IL-6、TGF-β促进肿瘤侵袭转移（如乳腺癌、胰腺癌）。

实验操作关键要点

WB检测（纤维化组织样本）：

样本处理：肝纤维化组织用含磷酸酶抑制剂（PhosSTOP+Na₃VO₄） 的RIPA裂解液冰上裂解30分钟，12000g离心15分钟取上清；

电泳条件：8% SDS-PAGE凝胶（因分子量较大），恒压80V浓缩胶、120V分离胶，转膜采用0.45μm PVDF膜（300mA恒流2.5小时）；

抗体孵育：一抗1:1000稀释（5% BSA+TBST），4℃孵育过夜，HRP二抗1:5000室温孵育1小时，曝光时间60秒（140kDa主条带需与非特异性条带区分）。

IHC-P组织定位：

抗原修复：EDTA缓冲液（pH9.0）高压修复10分钟（石蜡切片），增强间质细胞信号；

阳性定位：肝组织汇管区成纤维细胞胞膜/胞质阳性，肿瘤组织癌周间质细胞弥漫性表达；

对照设置：PDGFRβ敲除小鼠组织（阴性对照）和正常大鼠肾皮质（阳性对照，肾小球系膜细胞阳性）。

IF双标实验（肿瘤-间质共培养模型）：

推荐与α-SMA抗体共染，使用Alexa Fluor 488（绿色，PDGFRβ）和594（红色，α-SMA），激光共聚焦观察CAF中二者共表达（促进肿瘤侵袭的分子表型）。

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