生物制药 HPLC 分析->N-糖样品前处理和分析->内切糖苷酶

AdvanceBio N-聚糖酶 (PNGase F)，≥ 2.5 U/mL. 酶（PNGase F 的重组形式）通过在最内侧 GlcNAc 和 Asn 之间裂解，释放完整 N-糖。以 5 瓶 GKE-5006A 提供（5 x 100 mU，≥ 2.5 U/mL，1 mmol/L EDTA）。包括 5x N-糖酶反应缓冲液、变性溶液、去垢剂溶液、5x N-糖酶 Tris 反应缓冲液（质谱应用）。 更高酶浓度 (≥ 10 U/mL) 的配方可能有助于天然消化，请参阅 GKE-5010B 或 GKE-5020B（不含 EDTA）。

pH 范围	7.5-9.5
体积	40 µL
分子量	~35,000 daltons
单位	500 mU
** pH 值	8.6
浓度	≥2.5 U/mL
酶单位定义	One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
酶应用	Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies Release of intact N-linked glycans from glycopeptides and glycoproteins Structure-function studies of N-glycosylated glycoproteins
酶来源	Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum [1]. Enzyme is also known asPNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
酶特异性	Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless α(1-3) core fucosylated, as in plant glycans and some insect glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. This product is Endo F free.
酶配方	20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA (pH 7.5)