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Filipin III
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Filipin III图片
包装与价格:
包装价格(元)
500ug电议
1mg电议
5mg电议

产品介绍
Filipin III 是 Filipin 的主要成分,Filipin 是一种 28 元环戊烯大环内酯类抗真菌抗生素,由 S.

Cell lines

ergosterol-containing ciliary membranes

Preparation method

This compound is soluble in DMSO. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

12.7 μM

Applications

Filipin induced lysis of lecithin-cholesterol and lecithin-ergosterol vesicles, but did not lyse vesicles prepared from lecithin alone and from mixtures of lecithin and epicholesterol, thiocholesterol, androstan-3/3-ol, or cholestanol. Filipin treatment of ergosterol-containing ciliary membranes produced annuli with mean diameter almost identical with that in filipin-treated lecithin-ergosterol vesicles.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

产品描述

Cholesterol is both an important structural component of cell membranes and an early intermediate in hormone and bile acid biosynthesis. The localization and measurement of cholesterol in cells is therefore of great interest. Filipin is the collective name given to four isomeric polyene macrolides isolated from cultures of S. filipinensis; Filipin III is the predominant isomer and the one used in most studies. Filipin binds to cholesterol in membranes, forming ultrastructural aggregates and complexes which can be visualized by freeze-fracture electron microscopy.[1],[2] The binding of cholesterol also decreases the intrinsic fluorescence of Filipin, and this property has also been used to detect cholesterol in membrane fractions.[3]

Reference:
[1]. Castanho, M.A., Coutinho, A., and Prieto, M.J. Absorption and fluorescence spectra of polyene antibiotics in the presence of cholesterol J. Biol. Chem. 267(1), 204-209 (1992).
[2]. Miller, R.G. Mini Review. The use and abuse of filipin to localize cholesterol in membranes Cell Biol. Int. Rep. 8(7), 519-535 (1984).
[3]. Severs, N.J., and Robenek, H. Detection of microdomains in biomembranes. An appraisal of recent developments in freeze-fracture cytochemistry Biochimica et Biophysica Acta 737, 373-408 (1983)