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Cyclofenil
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Cyclofenil图片
CAS NO:2624-43-3
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
50mg电议
100mg电议
200mg电议
500mg电议

产品介绍
Cyclofenil 是一种选择性雌激素受体 (estrogen receptor) 调节剂和排卵诱导剂。Cyclofenil 对 Vero 细胞中的登革热病毒复制具有抑制作用,EC50为 1.62 μM。 Cyclofenil 具有抗登革热病毒的活性。
生物活性

Cyclofenil is a selectiveestrogen receptormodulator and an ovulation-inducing agent. Cyclofenil shows an inhibitory effect ondengue virusreplication in Vero cells with anEC50of 1.62 μM. Cyclofenil has anti-dengue-virus activity[1][2].

IC50& Target

Estrogen receptor[1]; Dengue virus[1]

体外研究
(In Vitro)

Cyclofenil shows an inhibitory effect on dengue virus (DENV) replication in mammalian cells but not in mosquito cells[1].
Cyclofenil also inhibits the replication of Zika virus. A time-of-addition assay suggested that Cyclofenil may interfere with two stages of the Dengue virus (DENV) life cycle: the translation-RNA synthesis and assembly-maturation stages. The level of intracellular infectious particles decreased more drastically after treatment with Cyclofenil than the viral RNA level do, indicating that the assembly-maturation stage might be the main target of Cyclofenil[1].

体内研究
(In Vivo)

The number of estrogen receptors α (ERα) and β (ERβ)-immunoreactive (-ir) cells is determined of each stage of the estrous cycle in intact rats. Additionally, the effects of blocking ERα and ERβ on ovulation rate on diestrus-2 or proestrus day through the microinjection of methyl-piperidino-pyrazole (MPP) or Cyclofenil in either side of preoptic-anterior hypothalamic area (POA-AHA) are evaluated. The number of ERα-ir and ERβ-ir cells in POA-AHA varied in each phase of estrous cycle. Either MPP or Cyclofenil in the right side of POA-AHA on diestrus-2 day reduced the ovulation rate, while at proestrus day it was decreased in rats treated in either side with MPP, and in those treated with Cyclofenil in the left side. MPP or Cyclofenil produced a decrease in the surge of luteinizing hormone levels (LH) and an increase in progesterone and follicle stimulating hormone (FSH)[2].

分子量

364.44

性状

Solid

Formula

C23H24O4

CAS 号

2624-43-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 100 mg/mL(274.39 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.7439 mL13.7197 mL27.4394 mL
5 mM0.5488 mL2.7439 mL5.4879 mL
10 mM0.2744 mL1.3720 mL2.7439 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.5 mg/mL (6.86 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (6.86 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。