| 生物活性 | Neuropeptide W-23 (human) (NPW-23), the active form of Neuropeptide W, is an endogenous agonist ofNPBW1 (GPR7)andNPBW2 (GPR8)[1]. |
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体外研究
(In Vitro) | Neuropeptide W-23 (human) (NPW-23) increases the ICa,Lin transfected human embryonic kidney 293 cells and VSMCs via GPR7[1].
Neuropeptide W-23 (human) increases the expression of pan phospho-PKC, intracellular diacylglycerol level, and the second messenger catalyzed by PLC[1].
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体内研究
(In Vivo) | Neuropeptide W-23 (human) (NPW-23) (0.3-3.0 nM; intracerebroventricular injection; 2 μL) increases total behavioral activity, including locomotion and grooming in conscious rats[2].
Neuropeptide W-23 (human) (NPW-23) (2-8 nM; i.c.v.; 10 μL) shows anorexigenic effect in rats[3].
| Animal Model: | Male Harlan Sprague-Dawley rats, 250–300 g[2] | | Dosage: | 0.3, 1.0 and 3.0 nM | | Administration: | Intracerebroventricular injection, 2 μL | | Result: | Caused significant increases in mean arterial pressure. Demonstrated a significant increase in total activity, ambulatory activity, and duration of stereotypy. |
| Animal Model: | Male Wistar rats weighing 250–300 g[3] | | Dosage: | 2, 4, 6 and 8 nM | | Administration: | Intra-cerebroventricular injection, 10 μL | | Result: | Decreased dark feeding and fasting-induced feeding, decreased feeding intake and weight gain. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Sealed storage, away from moisture | Powder | -80°C | 2 years | | -20°C | 1 year |
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| Solvent & Solubility | In Vitro: H2O Peptide Solubility and Storage Guidelines: 1. Calculate the length of the peptide. 2. Calculate the overall charge of the entire peptide according to the following table: | | Contents | Assign value | | Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 | | Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 | | Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution: | Overall charge of peptide | Details | | Negative (<0) | 1. Try to dissolve the peptide in water first.
2. If water fails, add NH4OH (<50 μL).
3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. | | Positive (>0) | 1. Try to dissolve the peptide in water first.
2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. | | Zero (=0) | 1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
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