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Procion Blue HB
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Procion Blue HB图片
CAS NO:12236-82-7
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
10mg电议
50mg电议
100mg电议

产品名称
蓝色染料
Reactive Blue 2
产品介绍
Procion Blue HB (Reactive Blue 2)是嘌呤能拮抗剂。
生物活性

Procion Blue HB (Reactive Blue 2) is apurinergicantagonist.

体外研究
(In Vitro)

Reactive Blue 2 is used as an ATP receptor antagonist and induces Ca2+oscillations in HeLa cells. Reactive Blue 2 enhances a Ca2+response to histamine that is linked to the PLC cascade. Reactive Blue 2 may activate the PLC cascade in an extracellular Ca2+-dependent manner and induce Ca2+oscillations[1]. The application of Reactive Blue 2 increases K+secretion in a dose-dependent manner, and this increase is characterized as a peak followed by a partial relaxation to a steady-state. Reactive Blue 2 has antagonistic activities at P2Y4, and the antagonist potency at P2Y4 paralleled the potency of K+ secretion[2].The anthraquinone dye reactive blue 2 is found to be a potent inhibitor of a protein kinase isolated and purified from thylakoids. The mode of inhibition is noncompetitive, with a Kiof 8 μM for the membrane-bound kinase, and 6 microM for the purified kinase. The inhibitor does not modify the substrate preference of the endogenous kinase and could be removed from the membrane by washing[3]. Reactive blue 2 selectively inhibits responses mediated via the P2ypurinoceptor, at least within a limited concentration range. In preparations where the tone has been raised with noradrenaline, ATP and 2-methylthio ATP, but not α,β-methylene ATP, produce relaxations of the vessel. These relaxations are inhibited in the presence of reactive blue 2[4]. Reactive blue 2, at concentrations of 0.3-10 μM blocks the ATP-induced oscillation in a concentration-dependent manner[5].

分子量

774.16

性状

Solid

Formula

C29H20ClN7O11S3

CAS 号

12236-82-7

中文名称

蓝色染料;普施安蓝 HB;活性蓝 2;汽巴蓝 F3GA

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO : ≥ 110 mg/mL(142.09 mM)

H2O : 10 mg/mL(12.92 mM;Need ultrasonic)

Ethanol : 1 mg/mL(1.29 mM;Need ultrasonic)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.2917 mL6.4586 mL12.9172 mL
5 mM0.2583 mL1.2917 mL2.5834 mL
10 mM0.1292 mL0.6459 mL1.2917 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。