CAS NO: | 12236-82-7 |
包装 | 价格(元) |
10 mM * 1 mL in DMSO | 电议 |
10mg | 电议 |
50mg | 电议 |
100mg | 电议 |
生物活性 | Procion Blue HB (Reactive Blue 2) is apurinergicantagonist. | ||||||||||||||||
体外研究 (In Vitro) | Reactive Blue 2 is used as an ATP receptor antagonist and induces Ca2+oscillations in HeLa cells. Reactive Blue 2 enhances a Ca2+response to histamine that is linked to the PLC cascade. Reactive Blue 2 may activate the PLC cascade in an extracellular Ca2+-dependent manner and induce Ca2+oscillations[1]. The application of Reactive Blue 2 increases K+secretion in a dose-dependent manner, and this increase is characterized as a peak followed by a partial relaxation to a steady-state. Reactive Blue 2 has antagonistic activities at P2Y4, and the antagonist potency at P2Y4 paralleled the potency of K+ secretion[2].The anthraquinone dye reactive blue 2 is found to be a potent inhibitor of a protein kinase isolated and purified from thylakoids. The mode of inhibition is noncompetitive, with a Kiof 8 μM for the membrane-bound kinase, and 6 microM for the purified kinase. The inhibitor does not modify the substrate preference of the endogenous kinase and could be removed from the membrane by washing[3]. Reactive blue 2 selectively inhibits responses mediated via the P2ypurinoceptor, at least within a limited concentration range. In preparations where the tone has been raised with noradrenaline, ATP and 2-methylthio ATP, but not α,β-methylene ATP, produce relaxations of the vessel. These relaxations are inhibited in the presence of reactive blue 2[4]. Reactive blue 2, at concentrations of 0.3-10 μM blocks the ATP-induced oscillation in a concentration-dependent manner[5]. | ||||||||||||||||
分子量 | 774.16 | ||||||||||||||||
性状 | Solid | ||||||||||||||||
Formula | C29H20ClN7O11S3 | ||||||||||||||||
CAS 号 | 12236-82-7 | ||||||||||||||||
中文名称 | 蓝色染料;普施安蓝 HB;活性蓝 2;汽巴蓝 F3GA | ||||||||||||||||
运输条件 | Room temperature in continental US; may vary elsewhere. | ||||||||||||||||
储存方式 | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) | ||||||||||||||||
溶解性数据 | In Vitro: DMSO : ≥ 110 mg/mL(142.09 mM) H2O : 10 mg/mL(12.92 mM;Need ultrasonic) Ethanol : 1 mg/mL(1.29 mM;Need ultrasonic) *"≥" means soluble, but saturation unknown. 配制储备液
* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |