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Cy5 DBCO chloride
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Cy5 DBCO chloride图片
CAS NO:2182601-72-3
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品介绍
Cy5 DBCO chloride 是一种叠氮化物反应探针,DBCO 分子的加入可以通过无铜 “Click Chemistry” 反应对叠氮化物标记的生物分子进行成像。
生物活性

Cy5 DBCO chloride is an azide reaction probe and the addition of DBCO molecules allows the imaging of azide-labelled biomolecules by a copper-free “Click Chemistry” reaction[1].

体外研究
(In Vitro)

Cy5 DBCO chloride (2-50 μM) can control the extent of labelling by varying the concentration of DBCO-Cy5 in a dose-dependent manner, with the amount of DBCO-Cy5 bound at the cell surface increasing with increasing DBCO-Cy5 concentration[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1.Incubate the cells at a density of 3×104cells in a cell culture dish for 3 days and then wash the cells twice with DPBS (pH 7.4). Incubate the cells according to your normal protocol.
2.Incubate the cells with Cy5 DBCO chloride at a final concentration of 20 μM for 1 h at 37℃.
3.After incubation, wash cells with DPBS (pH 7.4) and fix with formaldehyde-glutaraldehyde combination fixative for 15 min at room temperature.
4.After fixation, wash cells twice with DPBS (pH 7.4). Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.

分子量

777.43

Formula

C50H53ClN4O2

CAS 号

2182601-72-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.