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Resazurin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Resazurin图片
CAS NO:550-82-3
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品名称
刃天青
Diazoresorcinol
产品介绍
Resazurin (Diazoresorcinol) 是一种水溶性的、无毒的、稳定的膜渗透性蓝色非荧光染料 (有微弱的荧光)。Resazurin 作为氧化还原指示剂,在活细胞中能被还原成粉色的具有高度荧光的 Resorufin (Ex=530-560 nm, Em=590 nm),从而用于细胞 (人、动植物、细菌和真菌) 活力、毒性、增殖、迁移和侵袭的检测。
生物活性

Resazurin (Diazoresorcinol) is a water-soluble, non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent). Resazurin is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal,bacterialand fungal)[1][2].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Cell viability[1][2]:
1. Defrost the Resazurin solution in a 37℃ water bath.
2. Place the cells on a 96-well plate, and washed by PBS (avoid light).
3. Remove the PBS wash, then add500 μL(1μg/mL) of Resazurin solution.
4. Place the plate, in the incubator for30 min(incubation time depends on cell type and cell number).
5. Measure Resorufin fluorescence (Ex=530-560 nm, Em=590 nm) using a microplate reader.

分子量

229.19

Formula

C12H7NO4

CAS 号

550-82-3

中文名称

刃天青

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.