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Swainsonine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Swainsonine图片
CAS NO:72741-87-8
包装与价格:
包装价格(元)
1mg电议
5mg电议
10mg电议
50mg电议

产品名称
Tridolgosir
产品介绍
Swainsonine (Tridolgosir) 是一种天然的吲哚里西啶生物碱,是一种有效且可逆的 α-甘露糖苷酶 (α-mannosidase) 抑制剂。Swainsonine 诱导细胞凋亡 (apoptosis)和细胞周期停滞在 G2/M 期。Swainsonine 具有抗肿瘤活性。
生物活性

Swainsonine (Tridolgosir) is an natural indolizidine alkaloid, a potent and reversibleα-mannosidaseinhibitor. Swainsonine inducesapoptosisand cell cycle arrest at G2/M phase. Swainsonine shows anti-tumor activity[1][2][3].

体外研究
(In Vitro)

Swainsonine (0-40 μM; 12 h) shows antiproliferative activity in U251 and LN444 cells[2].
Swainsonine (30 μM; 12 h) induces apoptosis and cell cycle arrest at G2/M phase, and decreases the expression of CyclinD1 and CDK4 protein levels, increases the expression of p16 protein level[2].
Swainsonine (30 μM; 12 h) reduces glioma cell migration and invasion[2].

Cell Viability Assay[2]

Cell Line:U251, LN444 cells
Concentration:0, 10, 20, 30, 40 μM
Incubation Time:12 h
Result:Inhibited cell viability in a dose-dependent manner, and shows no cytotoxicity in normal brain cells.

Cell Cycle Analysis[2]

Cell Line:U251 cells
Concentration:30 μM
Incubation Time:12 h
Result:Induced cell cycle arrest at G2/M phase.

Apoptosis Analysis[2]

Cell Line:U251 cells
Concentration:30 μM
Incubation Time:12 h
Result:Induced apoptosis with the cleaved-Caspase-3 and cleaved-Caspase-9 expression were obviously increased.
体内研究
(In Vivo)

Swainsonine (1 mg/kg; i.p.; twice daily for ten days) and cisplatin increases the anti-tumor efficacy in mouse[3].

Animal Model:Eighty-seven sixty-day-old male C57BL/6 mice[3]
Dosage:1 mg/kg (cisplatin; 0.25 mg/kg, i.p., every other day for 10 days)
Administration:I.p.; twice daily for ten days
Result:Showed a significant reduction in ascites volume of 63.5% was observed in mice and a significant reduction in the percentage of cells in the G0/G1, S and G2 phases.
分子量

173.21

性状

Solid

Formula

C8H15NO3

CAS 号

72741-87-8

中文名称

苦马豆碱

结构分类
  • Alkaloids
  • Pyrrole Alkaloids
  • Alkaloids
  • Piperidine Alkaloids
来源
  • Plants
  • Leguminosae
  • Sphaerophysa salsula
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 10 mg/mL(57.73 mM;Need ultrasonic and warming)

Ethanol : 10 mg/mL(57.73 mM;Need ultrasonic and warming)

H2O : 3.57 mg/mL(20.61 mM;ultrasonic and warming and heat to 60℃)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM5.7733 mL28.8667 mL57.7334 mL
5 mM1.1547 mL5.7733 mL11.5467 mL
10 mM0.5773 mL2.8867 mL5.7733 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。