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Cycleanine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Cycleanine图片
CAS NO:518-94-5
包装与价格:
包装价格(元)
5mg电议
10mg电议
50mg电议
100mg电议

产品名称
轮环藤碱
产品介绍
Cycleanine 是一种高效的血管选择性Ca- 拮抗剂。Cycleanine 具有缓解疼痛、肌肉松弛和抗炎作用。Cycleanine 具有通过凋亡通路发挥抗卵巢癌作用的潜力。
生物活性

Cycleanine is a potent vascular selectiveCalciumantagonist. Cycleanine has analgesic, muscle relaxant and anti-inflammatory activities. Cycleanine has potential for anti-ovariancanceracting through theapoptosispathway[1][2].

IC50& Target

L-type calcium channel

 

体外研究
(In Vitro)

Cycleanine inhibits L-type Ca-current (ICaL) of single rat ventricular cardiomyocytes in a voltage- and frequency-dependent manner[1].
Cycleanine shows modestly less potency against human OSE cells (normal) than the cancer cells[2].
Cycleanine (20 μM; 48 hours) exhibits cytotoxicity for Ovcar-8, A2780, Igrov-1, and Ovcar-4 cell lines with IC50s ranging from 7 to 14 μM[2].
Cycleanine (20 μM; 24 hours) results in significant PARP cleavage (a marker of apoptosis)[2].
Cycleanine (20 μM; 48 hours) causes a significant increase of the population of both early and late apoptotic cells[2].

Cell Cytotoxicity Assay[2]

Cell Line:Ovcar-8 cells, A2780 cells, Igrov-1 cells, Ovcar-4 cells
Concentration:20 μM
Incubation Time:48 hours
Result:Exhibited cytotoxicity for Ovcar-8, A2780, Igrov-1, and Ovcar-4 cell lines with IC50values of 10 μM, 7.6 μM, 14 μM, 7.2 μM, respectively.

Western Blot Analysis[2]

Cell Line:Ovcar-8 cells
Concentration:20 μM
Incubation Time:24 hours
Result:Induced 1.1-fold increase in PARP-1 cleavage compared with carboplatin.

Apoptosis Analysis[2]

Cell Line:Ovcar-8 cells
Concentration:20 μM
Incubation Time:48 hours
Result:Caused a significant increase of the population of both early and late apoptotic cells.

Cell Cycle Analysis[2]

Cell Line:Ovcar-8 cells
Concentration:20 μM
Incubation Time:48 hours
Result:Increased the percentage of Ovcar-8 cells in subG1.
体内研究
(In Vivo)

Cycleanine inhibits the KCl-induced contraction of rabbit aortic rings with an IC50of 0.8 nM[1].

分子量

622.75

性状

Solid

Formula

C38H42N2O6

CAS 号

518-94-5

中文名称

轮环藤碱

结构分类
  • Alkaloids
  • Alkaloid Dimers
来源
  • Plants
  • Menispermaceae
  • Cissampelos pareira
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro: 

DMSO : 5 mg/mL(8.03 mM;ultrasonic and warming and heat to 60℃)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.6058 mL8.0289 mL16.0578 mL
5 mM0.3212 mL1.6058 mL3.2116 mL
10 mM---------
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。