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Rifamycin S
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Rifamycin S图片
CAS NO:13553-79-2
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
25mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品名称
利福霉素S
产品介绍
Rifamycin S 是一种醌和抗革兰氏阳性细菌 (包括MRSA) 的抗生素。Rifamycin S 是涉及两个电子的可逆氧化还原系统的氧化形式。Rifamycin S 可以产生活性氧 (ROS) 并抑制微粒体脂质过氧化,并可用于肺结核和麻风病的研究。
生物活性

Rifamycin S, a quinone, is anantibioticagainstGram-positive bacteria(includingMRSA). Rifamycin S is the oxidized forms of a reversible oxidation-reduction system involving two electrons. Rifamycin S generatesreactive oxygen species(ROS)and inhibits microsomal lipid peroxidation. Rifamycin S can be used for tuberculosis and leprosy[1][2][3].

IC50& Target

Gram-positive bacteria[3]
Reactive oxygen species (ROS)[1]

体外研究
(In Vitro)

The inhibition of bacterial growth by Rifamycin SV is due to the production of active species of oxygen resulting from the oxidation-reduction cycle of Rifamycin SV in the cells. The aerobic oxidation of Rifamycin SV to Rifamycin S is induced by metal ions, such as Mn2+, Cu2+, and Co2+. The most effective metal ion is Mn2+[2].

体内研究
(In Vivo)

Rat liver sub-mitochondrial particles also generated hydroxyl radical in the presence of NADH and Rifamycin S. NADH dehydrogenase (complex I) as the major component involved in the reduction of Rifamycin S. Compared to NADPH, NADH is almost as effective (Rifamycin S) in catalyzing the interactions of these antibiotics with rat liver microsomes. Rifamycin S is shown to be readily reduced to Rifamycin SV, the corresponding hydroquinone by Fe(II). Rifamycin S forms a detectable Fe(II)-(Rifamycin S)3 complex. The Fe:ATP induced lipid peroxidation is completely inhibited by Rifamycin S. Rifamycin S can interact with rat liver microsomes to undergo redox-cycling, with the subsequent production of hydroxyl radicals when iron complexes are present[1].

分子量

695.75

性状

Solid

Formula

C37H45NO12

CAS 号

13553-79-2

中文名称

利福昔明EP杂质E (Rifamycin S)

结构分类
  • Quinones
  • Naphthalene Quinones
  • Phenols
  • Monophenols
来源

Curvularia lunata

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 100 mg/mL(143.73 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.4373 mL7.1865 mL14.3730 mL
5 mM0.2875 mL1.4373 mL2.8746 mL
10 mM0.1437 mL0.7186 mL1.4373 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: 2.5 mg/mL (3.59 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (3.59 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
*以上所有助溶剂都可在本网站选购。