Schisandrin (Schizandrin) 是一种从Schisandra chinensis的果实中分离出来的联苯环辛烯类木脂素。Schisandrin 具有抗氧化，保肝，抗癌和抗炎的活性。Schisandrin 还可以逆转大鼠的记忆障碍。
| 生物活性 | Schisandrin (Schizandrin), a dibenzocyclooctadiene lignan, is isolated from the fruit ofSchisandra chinensisBaill. Schisandrin exhibits antioxidant, hepatoprotective, anti-cancer and anti-inflammatory activities. Schisandrin also can reverses memory impairment in rats[1][2][3]. |
体外研究
(In Vitro) | Schisandrin (10-100 μM; pretreated for 2 h) inhibits LPS-stimulated NO production, iNOS protein and mRNA expression in a dose-dependent manner in RAW 264.7 cells[1].
Schisandrin (25-100 μM; pretreated for 2 h) inhibits prostaglandin E2 production, COX-2 protein and mRNA expression in LPS-stimulated RAW 264.7 macrophages[1].
Schisandrin inhibits the SK-HEP-1, SNU-638, and T47D cells proliferation, with IC50s of 42.0, 53.1, and 40.0 μM, respectively[3].
Schisandrin (10-60 μM; 48 h) enhances Doxorubicin -induced apoptosis and selectively reverses MCF-7/DOX resistance[4].
Cell Viability Assay[1] | Cell Line: | RAW 264.7 macrophages | | Concentration: | 12.5, 25, 50, 100 μM | | Incubation Time: | Pretreated for 2 h and then incubated with LPS (1 μg/mL) for 18 h | | Result: | Markedly decreased iNOS protein expression a dose-dependent manner.
Significantly inhibited COX-2 protein expression. |
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体内研究
(In Vivo) | Schisandrin (10-100 mg/kg; a single i.p.) inhibits acetic acid-induced peritoneal vascular permeability and reduces the carrageenan-induced paw edema of mice[1].
Schisandrin (1-10 mg/kg; a single p.o.) significantly reverses the Scopolamine-induced impairment of spatial memory and passive avoidance response, and enhances tremors induced by Oxotremorine in rats[2].
| Animal Model: | Male ICR mice (4-weeks) were treated with λ-carrageenan by intraperitoneal injection into the right hind paw[1] | | Dosage: | 100, 200 mg/kg | | Administration: | A single i.p. | | Result: | Inhibited paw edema by 33.43 % (100 mg/kg) and 57.38 % (200 mg/kg) at 3 h. |
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| 来源 | - Plants
- Schisandraceae
- Schisandra chinensis(Turcz.) Baill.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : 1 mg/mL(2.31 mM;Need ultrasonic) 配制储备液 | 1 mM | 2.3121 mL | 11.5604 mL | 23.1209 mL | | 5 mM | --- | --- | --- | | 10 mM | --- | --- | --- |
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储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
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此方案可获得 ≥ 0.28 mg/mL (0.65 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 2.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 *以上所有助溶剂都可在本网站选购。
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