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Kanamycin Sulfate
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Kanamycin Sulfate图片
CAS NO:25389-94-0
包装与价格:
包装价格(元)
10 mM * 1 mL in Water电议
200mg电议
1 g电议
5 g电议
10 g电议
50 g电议

产品名称
硫酸卡那霉素
Kanamycin A sulfate
产品介绍
Kanamycin (Kanamycin A) sulfate 是一种口服有效的抗菌剂 (革兰氏阴性/阳性细菌),可通过与 70 S 核糖体亚单位结合,抑制转位并导致编码错误。Kanamycin sulfate 对M. tuberculosis(敏感或耐药) 以及K. pneumonia均具有较好的抑制活性,可用于肺结核和肺炎的研究。
生物活性

Kanamycin (Kanamycin A) sulfate is an orally active antibacterial (gram-negative/positive bacteria) agent, inhibits translocation and causes misencoding by binding to the 70 S ribosomal subunit. Kanamycin sulfate shows good inhibitory activity to bothM. tuberculosis(sensitive and drug-resistant ) andK. pneumonia, which can be used in studies of tuberculosis and pneumonia[1][2][3][4].

体外研究
(In Vitro)

Kanamycin sulfate (0.1-100 μg/mL; 2 weeks) exhibits good antibacterial activity (MIC=1-5 μg/mL) to various strains ofmycobacteriain vitro[1].

Cell Viability Assay[1]

Cell Line:mycobacteriaH37Rv, H2, H37RvR-PAS, Ravenel, Kirchbergand and BCG.
Concentration:0.1-100 μg/mL
Incubation Time:2 weeks
Result:Showed good antibacterial activity to various strains ofmycobacteria(H37Rv, H2, H37RvR-PAS, Ravenel, and BCG) with MICs were 1 μg/mL and 5 μg/mL for strain of Kirchbergand.
体内研究
(In Vivo)

Kanamycin sulfate (2, 4 mg/kg; s.c.; once daily, 6 times a week for 3 weeks) inhibits growth ofbovine tubercle bacilliin lung and spleen of mice[1].
Kanamycin sulfate (1.25, 5 mg/kg; s.c.; single (at 3 h after infection)) inhibits the multiplication ofK. pneumoniaDT-S in lung, trachea, and blood of mice and in proportion to the dose administration, and also increases the survival rate of mice[2].

Animal Model:Inbred strain normal mice (14-16 g;bovine tubercle bacilli(Ravenel strain) infected model)[1].
Dosage:2, 4 mg/kg
Administration:Subcutaneous injection; once daily, 6 times a week for 3 weeks.
Result:Exerted a marked effect to inhibit the multiplication of thetuberculosisin vivo, especially in the lung of mice.
Animal Model:Slc:ICR male mice (4-week-old; 18-24 g;K. pneumoniaDT-S infection model (by the aerosol method))[2].
Dosage:1.25, 5 mg/kg
Administration:Subcutaneous administration; single (at 3 h after infection).
Result:Suppressed the growth ofK. pneumoniaDT-S in lung, trachea, and blood in proportion to the dose administration.
Resulted in 90% survival at 6 days after infection (negative control group: all died within 4 days), and cleared theK. pneumoniaDT-S from lung, trachea, and blood of mice within 48 h (when dosage at 5 mg/kg).
Clinical Trial
分子量

582.58

性状

Solid

Formula

C18H38N4O15S

CAS 号

25389-94-0

中文名称

硫酸卡那霉素;单硫酸卡那霉素;硫酸卡那辛

结构分类
  • Antibiotics
  • Other Antibiotics
来源

Streptomyces kanamyceticus

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : ≥ 25 mg/mL(42.91 mM)

DMSO :< 1 mg/mL(insoluble or slightly soluble)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.7165 mL8.5825 mL17.1650 mL
5 mM0.3433 mL1.7165 mL3.4330 mL
10 mM0.1717 mL0.8583 mL1.7165 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 50 mg/mL (85.83 mM); Clear solution; Need ultrasonic

  • 2.

    请依序添加每种溶剂: Saline

    Solubility: 50 mg/mL (85.83 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。