BNS-22

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BNS-22

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

1151668-24-4

包装与价格：

包装	价格(元)
100mg	电议
250mg	电议
500mg	电议

产品介绍

BNS-22 是一种 DNA 拓扑异构酶 II (TOP2) 催化抑制剂，作用于人的 TOP2α 和 TOP2β 的IC₅₀值分别是 2.8 μM 和 0.42 μM。BNS-22 可诱导分裂异常，具有抗增殖活性。

生物活性

BNS-22 is a DNAtopoisomeraseII (TOP2) catalytic inhibitor with theIC₅₀values of 2.8 μM and 0.42 μM for human TOP2α and TOP2β, respectively. BNS-22 induces abnormal division and has anti-proliferative activity^[1].

IC₅₀& Target^[1]

topoisomerase II alpha

2.8 μM (IC₅₀, in human)

topoisomerase II beta

0.42 μM (IC₅₀, in human)

体外研究
(In Vitro)

BNS-22 (0-30 μM, 24-48 h) can inhibit cell growth and influence on cell cycle progression of the human cervical epidermoid carcinoma cell line HeLa in a dose-dependent manner^[1].
BNS-22 (0-30 μM, 0-6 h) does not induce DNA damage and has an antagonistic effect on TOP2 toxin-mediated DNA damage^[1].

Cell Proliferation Assay^[1]

Cell Line:	HeLa cell lines
Concentration:	0-30 μM
Incubation Time:	24 h, 48 h
Result:	Inhibited cell growth with the IC₅₀value of 4.9 μM after 24 hours and 1.0 μM after 48 hours.

Cell Cycle Analysis^[1]

Cell Line:	HeLa cell lines
Concentration:	0-30 μM
Incubation Time:	24 h
Result:	Increased number of cells in G2/M phase. Disrupted mitotic spindle formation and induced polyploid cell formation at 3 μM.

Western Blot Analysis^[1]

Cell Line:	HeLa cell lines
Concentration:	0-30 μM
Incubation Time:	0, 30 min, 1 h, 2 h, 4 h, 6 h
Result:	Decreased etoposide-induced γ-H2AX expression and decreased total cellular TOP2β levels, but not TOP2α levels.

分子量

407.46

Formula

C₂₄H₂₅NO₅

CAS 号

1151668-24-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.