您好,欢迎来到化工原料网! [登录] [免费注册]
化工原料网
位置:首页 > 产品库 > Ghrelin(rat)
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
Ghrelin(rat)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Ghrelin(rat)图片
包装:1mg
市场价:3318元

产品介绍

Ghrelin (rat), a growth hormone-releasing peptide first discovered in rat stomach in 1999, is a ligand for the growth hormone secretagogue receptor.

Cell lines

RP adipocytes

Preparation Method

After isolation, adipocytes were seeded (800 µl of suspension per well) onto 24 well plates; then, 200 µl of the following test solutions were added: culture medium either alone (basal) or containing insulin; dexamethasone phosphate, Ghrelin (rat)(0.001-1 nM), desacyl Ghrelin (rat), cycloheximid, actinomycin D , Ghrelin (rat) antagonist or, when appropriate, different combinations of these substances.

Reaction Conditions

0.001-1 nM for 0.5-48h at 37°C

Applications

Retroperitoneal (RP) adipocytes were cultured in the absence or presence of either Ghrelin (rat) or desacyl Ghrelin (rat) and in combination with either inhibitors of protein synthesis, insulin, dexamethasone (DXM), or GHSR1a antagonist. The results indicate that both Ghrelin (rat) forms possess a direct leptin-releasing activity (LRA) on RP adipocytes and significantly enhanced adipocyte ob mRNA expression.

Animal models

Male Sprague-Dawley rats (M&B, Ry, Denmark) 12-16 wk of age and weighing ~300 g

Preparation Method

Ghrelin (rat) (100 nmol/kg) dissolved in saline, was injected into the catheterized femoral vein. The influence of both exogenously administered Ghrelin (rat) and antagonism of endogenous Ghrelin (rat) levels (by GHRP-6 administration) was investigated in normal-fed rats.

Dosage form

100 nmol/kg Ghrelin (rat)(I.V)

Applications

Ghrelin (rat) and the GHS-R1α receptor antagonist GHRP-6 were injected intravenously in rats followed by blood flow measurements using a microsphere technique. Ghrelin decreased, while GHRP-6 in fasted, but not fed, rats selectively increased islet blood flow fourfold.

产品描述

Ghrelin (rat), a growth hormone-releasing peptide first discovered in rat stomach in 1999, is a ligand for the growth hormone secretagogue receptor[1,2].

Retroperitoneal (RP) adipocytes were cultured in the absence or presence of either Ghrelin (rat) or desacyl Ghrelin (rat) and in combination with either inhibitors of protein synthesis, insulin, dexamethasone (DXM), or GHSR1a antagonist. The results indicate that both Ghrelin (rat) forms possess a direct leptin-releasing activity (LRA) on RP adipocytes and significantly enhanced adipocyte ob mRNA expression[4].

Ghrelin (rat) and the GHS-R1α receptor antagonist GHRP-6 were injected intravenously in rats followed by blood flow measurements using a microsphere technique. Ghrelin (rat) decreased, while GHRP-6 in fasted, but not fed, rats selectively increased islet blood flow fourfold[3]. The initial electrophysiological results displayed that ex vivo administration of Ghrelin (rat) increased NAc shell output in brain slices from drug- and training-naIve rats. In rats with an acquired skilled reach performance, acute as well as repeated treatment with a Ghrelin (rat) receptor (GHSR-1 A) antagonist decreased the number of sucrose pellets consumed[5]. Ghrelin (rat) administration attenuated sepsis symptoms induced by CLP. Blood flow in the stomach greater curvature was significantly higher in the CLP induced sepsis group rats compared with the sham operation group, whereas there was no difference between the CLP group treated with Ghrelin (rat) and the sham rats. Ghrelin (rat) administration also reduced the secretion of pro inflammatory cytokines compared with the CLP induced sepsis group rats[6]. Ghrelin (rat) signaling increases the reward from social interaction in a manner that reflects the degree of divergence in body weight between the social pair[7].

References:
[1]. Tokudome T, Kangawa K. Physiological significance of ghrelin in the cardiovascular system. Proc Jpn Acad Ser B Phys Biol Sci. 2019;95(8):459-467. doi: 10.2183/pjab.95.032. PMID: 31611501; PMCID: PMC6819151.
[2]. Kojima M, Hosoda H, et,al.in is a growth-hormone-releasing acylated peptide from stomach. Nature. 1999 Dec 9;402(6762):656-60. doi: 10.1038/45230. PMID: 10604470.
[3]. Drott CJ, FranzEn P, et,al. Ghrelin in rat pancreatic islets decreases islet blood flow. Am J Physiol Endocrinol Metab. 2019 Jul 1;317(1):E139-E146. doi: 10.1152/ajpendo.00004.2019. Epub 2019 May 7. PMID: 31063397.
[4]. Giovambattista A, Gaillard RC, et,al.Ghrelin gene-related peptides modulate rat white adiposity. Vitam Horm. 2008;77:171-205. doi: 10.1016/S0083-6729(06)77008-X. PMID: 17983857.
[5]. Vestlund J, Bergquist F, et,al. Ghrelin signalling within the rat nucleus accumbens and skilled reach foraging. Psychoneuroendocrinology. 2019 Aug;106:183-194. doi: 10.1016/j.psyneuen.2019.04.008. Epub 2019 Apr 8. PMID: 30999229.
[6]. Li B, Lin Q, et,al.Ghrelin regulates sepsis?induced rat acute gastric injury. Mol Med Rep. 2019 Jun;19(6):5424-5432. doi: 10.3892/mmr.2019.10208. Epub 2019 Apr 30. PMID: 31059095; PMCID: PMC6522907.
[7]. SchEle E, Pfabigan DM, et,al. Ghrelin Induces Place Preference for Social Interaction in the Larger Peer of a Male Rat Pair. Neuroscience. 2020 Nov 1;447:148-154. doi: 10.1016/j.neuroscience.2020.01.027. Epub 2020 Feb 5. PMID: 32032669.