您好,欢迎来到化工原料网! [登录] [免费注册]
化工原料网
位置:首页 > 产品库 > Dalazatide
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
Dalazatide
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
CAS NO:1081110-69-1
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品名称
ShK-186
产品介绍
Dalazatide (ShK-186) 是一种特异性的Kv1.3钾通道肽抑制剂。Dalazatide 可用于自身免疫性疾病,如多发性硬化症 (MS)、红斑狼疮、银屑病、类风湿性关节炎、1 型糖尿病和炎症性肠病的研究。
生物活性

Dalazatide (ShK-186) is a specificKv1.3potassium channelpeptide inhibitor. Dalazatide can be used in the study of autoimmune diseases such as multiple sclerosis (MS), lupus erythematosus, psoriasis, rheumatoid arthritis, type 1 diabetes and inflammatory bowel disease[1][2][3].

体外研究
(In Vitro)

Dalazatide (ShK-186) (0-1000 pM) 以剂量依赖的方式阻断卵巢特异性 GFP+ 效应记忆 T (Tem) 细胞中的 Kv1.3 电流,Kd为 65 ± 5 pM[3]
Dalazatide (0-100 nM; 3 days) 以剂量依赖的方式抑制 CCR7T 细胞增殖[3]
Dalazatide (100 nM; 30 min) 通过抑制钙信号通路固定炎症部位的效应记忆 T (Tem) 细胞,从而阻止 β1 整合素激活[3]

Cell Proliferation Assay[3]

Cell Line:CCR7T cell[3]
Concentration:0-100 nM
Incubation Time:3 days
Result:Inhibited cell proliferation with an IC50of 180 ± 37 pM.
体内研究
(In Vivo)

Dalazatide (ShK-186) (100 μg/kg; s.c.; once) 在大鼠中抑制延迟型超敏反应,并抑制 Tem 细胞的体内运动和激活[3]

Animal Model:Lewis rats rats, delayed-type hypersensitivity (DTH) model[3]
Dosage:100 μg/kg
Administration:Subcutaneous injection, once
Result:Reduced DTH at all time points compared to rats given saline injections. Suppressed the proliferation of the Tem cells.
Clinical Trial
分子量

4442.08

Formula

C184H296N57O55PS7

CAS 号

1081110-69-1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

 ContentsAssign value
Acidic amino acidAsp (D), Glu (E), and the C-terminal -COOH.-1
Basic amino acidArg (R), Lys (K), His (H), and the N-terminal -NH2+1
Neutral amino acidGly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)0

3.  Recommended solution:

Overall charge of peptideDetails
Negative (<0)1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.