In vitro activity: Adenosine dialdehyde acts as an indirect inhibitor that can be incorporated by cells. The inhibition of S-adenosyl-L-homocystein hydrolase by ADOX can lead to the accumulation of S-adenosyl-L-homo cystein (Adoicy), a product inhibitor of methyltransferases that utilize S-adenosyl-L-methionine (AdoMet) as the methyl group donor. ADOX inhibited the Tax-activated NF-κB pathway, resulting in reactivation of p53 and induction of p53 target genes.

Kinase Assay:

Cell Assay: Hela cells are grown in MEM medium supplemented with 10% fetal bovine serum at 37 ℃ in a 5% CO2 incubator. Treatment of cells with ADOX for various time periods was performed. The cells are harvested and washed with phosphate-buffered saline, resuspended in buffer A (phosphate-buffered saline with 5% glycerol, 1 mM sodium EGTA, 1 mM dithiothreitol, 0.5% Triton X-100 and Complete protease inhibitor cocktail).