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Dnp-PLGLWA-DArg-NH2 TFA
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Dnp-PLGLWA-DArg-NH2 TFA图片
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品介绍
Dnp-PLGLWA-DArg-NH2 TFA 是 MMP-1 和 MMP-9 的荧光底物。Dnp-PLGLWA-DArg-NH2 TFA 可用来定量 MMPs 的活性 (Ex=280 nm, Em=360 nm)。
生物活性

Dnp-PLGLWA-DArg-NH2 TFA is a fluorogenic substrate forMMP-1andMMP-9. Dnp-PLGLWA-DArg-NH2 TFA can be used to quantify the activity of MMPs (Ex=280 nm, Em=360 nm)[1][2].

IC50& Target[1]

MMP-1

 

MMP-9

 

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[2].
1. Enzyme activity assays are performed in 50 mmol/l Tris-HCl buffer, pH 7.5, 0.15 mol/l NaCl, 10 mmol/l CaCl2, 0.02% TNC buffer containing 0.05% Brij 35 and 50 μM ZnSO4.
2. Dnp-PLGLWA-DArg-NH2 TFA. Each fraction is incubated with 1 μM substrate at 37℃ for 20 h.
3. Stop the reaction by the addition of 3% acetic acid.
4. Measure the fluorescence using wavelengths of 280 nm (excitation) and 360 nm (emission) with a fluorescence reader.

分子量

1091.10

Formula

C47H65F3N14O13

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.