Nudol 是一种具有抗癌活性的菲 (phenanthrene) 化合物。Nudol 抑制细胞增殖，诱导细胞凋亡 (apoptosis)。Nudol 抑制 MMP-2 和 MMP-9 活性 (Ki值分别为 988.9 nM 和 1.76 μM)。Nudol 可用于骨肉瘤等癌症的研究。
| 生物活性 | Nudol is a phenanthrene compound that has anti-cancer activity. Nudol inhibits cell proliferation, induces cellapoptosis. Nudol inhibitsMMP-2MandMMP-9activity (Ki: 988.9 nM, 1.76 μM, respectively). Nudol can be used in the research of cancers, such as osteosarcoma[1][2]. |
| IC50& Target[2] | MMP-2 988.9 nM (Ki) | MMP-9 1.76 μM (Ki) |
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体外研究
(In Vitro) | Nudol (0-40 μM, 24-72 h) decreases cell viability in several cancer cell lines[1].
Nudol (0-20 μM, 24/48 h) suppresses the cell migration and causes cell cycle arrest at G2/M phase in U2OS cells[1].
Nudol (20 μM, 48 h) induces cell apoptosis through the caspase-dependent pathway in U2OS cells[1].
Nudol (compound 4) inhibits MMP-2 and MMP-9 activity with Kivalues of 988.9 nM and 1.76 μM respectively[2].
Cell Viability Assay[1] | Cell Line: | U2OS, MG63, MDA-MB-231, MCF-7, and A549 cells | | Concentration: | 0, 10, 20, 30, 40 μM | | Incubation Time: | 24, 48, and 72 h | | Result: | Decreased the cell viability of osteosarcoma cells in dose- and time-dependent manners. |
Cell Cycle Analysis[1] | Cell Line: | U2OS cells | | Concentration: | 0, 5, 10, 20 μM | | Incubation Time: | 24, 48, and 72 h | | Result: | Triggered G2/M phase arrest by decreasing cell cycle-related proteins (CDK1, CDK2, CDK4, and CDK10). |
Western Blot Analysis[1] | Cell Line: | U2OS cells | | Concentration: | 0, 5, 10, 20, 40 μM | | Incubation Time: | 24, 48, and 72 h | | Result: | Increased the protein level of cytochrome c.
Down-regulated anti-apoptotic Bcl-2, accompanied by an increased level of pro-apoptotic Bax. |
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| 来源 | - Plants
- Orchidaceae
- Epigeneium rotundatum(Lindl.) Summerh.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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