Proteasome-activating peptide 1 TFA 是一种肽，是一种有效的蛋白酶体激活剂。Proteasome-activating peptide 1 TFA 可增加胰凝乳蛋白酶样蛋白酶体催化活性，从而提高体外和培养中的蛋白水解率。Proteasome-activating peptide 1 TFA 可防止肌萎缩侧索硬化症细胞模型中的蛋白质聚集。
| 生物活性 | Proteasome-activating peptide 1 TFA is a peptide and a potentproteasomeactivator. Proteasome-activating peptide 1 TFA increases the chymotrypsin-like proteasomal catalytic activity and, consequently, proteolytic rates both in vitro and in culture. Proteasome-activating peptide 1 TFA prevents protein aggregation in a cellular model of amyotrophic lateral sclerosis[1]. |
体外研究
(In Vitro) | Proteasome-activating peptide 1 (TFA) (0-100 μM, 2 h) increases ChT-L catalytic activity of the 20S proteasome in a dose-dependent manner[1].
Proteasome-activating peptide 1 (TFA) (50 μM, 24 h) induces the degradation of SOD1, promotes a decrease of oxidized proteins[1].
Proteasome-activating peptide 1 (TFA) (0-100 μM) protects fibroblast and SH-SY5Y cells from oxidative stress[1].
Proteasome-activating peptide 1 (TFA) only affects the free 20S proteasomal pool[1].
Proteasome-activating peptide 1 (TFA) promotes proteasomal gate opening[1].
Cell Viability Assay | Cell Line: | Human neuroblastoma (SH-SY5Y) and fibroblast cells[1] | | Concentration: | 0, 50, 100 μM (in DMEM without fetal bovine serum followed by the addition of H2O2 (500 or 750 μM) or water (0.4%)) | | Incubation Time: | 30 min or 2 h | | Result: | Promoted long-term proteasomal activation through interaction with the 20S core particle, increased ChT-L activity of the 20S proteasome in a dose-dependent manner, and did not reduce cell fibroblast viability. |
Western Blot Analysis | Cell Line: | SH-SY5YG93Acells[1] | | Concentration: | 50 μM | | Incubation Time: | 24 h | | Result: | Increased the degradation of soluble SOD1, prevented the formation of SOD1-insoluble aggregates, and decreased the oxidized proteins in the total intracellular content. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Sealed storage, away from moisture | Powder | -80°C | 2 years | | -20°C | 1 year |
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| 溶解性数据 | In Vitro: H2O : ≥ 50 mg/mL(30.64 mM) *"≥" means soluble, but saturation unknown. 配制储备液 | 1 mM | 0.6127 mL | 3.0637 mL | 6.1274 mL | | 5 mM | 0.1225 mL | 0.6127 mL | 1.2255 mL | | 10 mM | 0.0613 mL | 0.3064 mL | 0.6127 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
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