Recombinant Proteinase K is aserine proteasethat cleaves the carboxy-terminated peptide bonds of aliphatic and aromaticamino acids. Recombinant Proteinase K can be used to digest proteins and remove contamination from nucleic acid preparations^[1].

Proteinase K digestion^[1]
1. Cells are resuspended in lysis bufferand finally lysed on ice for 15 minutes.
2. The crude lysates are obtained by centrifugation at 4℃ for 15 minutes. Proteinase K is added to a 20 μl aliquot to get the final concentrations of 2, 4 and 8 μg/ml. Reactions are carried out at 37℃ for 30 minutes.

Solid

重组蛋白酶 K

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)