Moclobemide (Ro111163) 是一种可透过血脑屏障的可逆的单胺氧化酶 (MAO-A) 抑制剂,对 hMAO-A 的IC50为 6.061 μM。Moclobemide 上调慢性应激小鼠海马祖细胞的增殖。
生物活性 | Moclobemide (Ro111163) is a brain-penetrant and reversiblemonoamine oxidase(MAO-A) inhibitor with anIC50of 6.061 μM for hMAO-A[1].Moclobemide up-regulates proliferation of hippocampal progenitor cells in chronically stressed mice. |
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体外研究 (In Vitro) | NMDA (600 μM for 3 days) inhibits the proliferation of PC12 cells.Moclobemide (2 and 10 μM) up-regulates the proliferation in NMDA-treated PC12 cells[2].
Cell Cycle Analysis[2] Cell Line: | PC12 cell line | Concentration: | Moclobemide (2 and 10 μM); N-methylaspartate (NMDA) (600 μM) | Incubation Time: | 3 days | Result: | Treatment with NMDA significantly reduced the percentage of S-phase, while the percentage of other cell cycle phases did not change notablely.However,the percentage of S-phase increased in the presence of Moclobemide. |
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体内研究 (In Vivo) | Moclobemide is a monoamine oxidase inhibitor and increases the levels of brain monoamines (such as 5-HT, norepinephrine).Moclobemide(40 mg/kg) is effective in animal behavior models[2].
Animal Model: | Chronically stressed male mice (18±2 g) of the Kunming strain[2] | Dosage: | 40 mg/kg | Administration: | I.p.; daily | Result: | BDNF level in the hippocampal subfields including subgranule zone decreased in stressed mice compared with normal control. Chronic treatment with Moclobemide could reverse these changes. |
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运输条件 | Room temperature in continental US; may vary elsewhere. |
储存方式 | Powder | -20°C | 3 years | | 4°C | 2 years | In solvent | -80°C | 6 months | | -20°C | 1 month |
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溶解性数据 | In Vitro: DMSO : 100 mg/mL(372.11 mM;Need ultrasonic) 配制储备液 1 mM | 3.7211 mL | 18.6053 mL | 37.2107 mL | 5 mM | 0.7442 mL | 3.7211 mL | 7.4421 mL | 10 mM | 0.3721 mL | 1.8605 mL | 3.7211 mL |
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以下溶剂前显示的百 分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂: 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.5 mg/mL (9.30 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (9.30 mM,饱和度未知) 的澄清溶液。 以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。 2. 请依序添加每种溶剂: 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 2.5 mg/mL (9.30 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (9.30 mM,饱和度未知) 的澄清溶液。 以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。 3. 请依序添加每种溶剂: 10% DMSO 90%corn oil Solubility: ≥ 2.5 mg/mL (9.30 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (9.30 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。 *以上所有助溶剂都可在本网站选购。
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