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TOFA
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
TOFA图片
CAS NO:54857-86-2
规格:≥98%
包装与价格:
包装价格(元)
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议
1g电议

产品介绍
理化性质和储存条件
Molecular Weight (MW) 324.45
Formula C19H32O4
CAS No. 54857-86-2
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: ≥ 34 mg/mL
Water: <1 mg/mL
Ethanol: <1 mg/mL
SMILES Code O=C(C1=CC=C(OCCCCCCCCCCCCCC)O1)O
Synonyms RMI14514; RMI-14514; MDL14514; RMI 14514; MD14514; MDL-14514
实验参考方法
In Vitro

In vitro activity: TOFA (5-tetradecyloxy-2-furoic acid) is cytotoxic to lung cancer cells NCI-H460 and colon carcinoma cells HCT-8 and HCT-15, with an IC50at approximately 5.0, 5.0, and 4.5 μg/mL, respectively. TOFA at 1.0–20.0 μg/mL effectively blocks fatty acid synthesis and induces cell death in a dose-dependent manner.TOFA is found to be cytotoxic to COC1 and COC1/DDP cells with IC50 values of ~26.1 and 11.6 μg/mL, respectively. TOFA inhibits the proliferation of the cancer cells examined in a time and dose dependent manner, arrests the cells in the G0/G1 cell cycle phase and induces apoptosis. Acetyl-CoA-carboxylase-α (ACCA) is a key enzyme in the regulation of fatty acids synthesis. Inhibition of ACCA by TOFA decreases fatty acid synthesis and induces caspase activation and cell death in most PCa cell lines.


Kinase Assay:


Cell Assay: NCI-H460, human lung cancer cells, and HCT-8 and HCT-15 cells (5,000/well) are seeded in 96-well plates overnight and then exposed to TOFA at indicated concentrations (0, 1, 5, 10, 20, 50 μg/mL) for 72 hours. Viable cells are detected using MTT assay.

In VivoTOFA inhibits COC1/DDP cell growth in ovarian tumor mouse xenografts. The tumor growth rate is signifi!cantly inhibited by TOFA compared with the DMSO treated control mice (1649±356.3 vs. 5128±390.4 mm3. No toxicity is observed in the heart, liver, spleen, lung, kidney and intestinal tissues. By inhibiting ACC, TOFA may be a promising small molecule agent for ovarian cancer therapy.
Animal model Mice
Formulation & Dosage 50 mg/kg; i.p.
References Biochem Biophys Res Commun. 2009 Jul 31;385(3):302-6. Mol Med Rep. 2013 Aug;8(2):373-8.Cancer Biol Ther. 2011 Jul 1;12(1):80-5.