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Prodigiosin hydrochloride
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Prodigiosin hydrochloride图片
CAS NO:56144-17-3
包装与价格:
包装价格(元)
100 μg电议
500 μg电议
1mg电议
5mg电议
10mg电议

产品名称
Prodigiosine hydrochloride
产品介绍
Prodigiosin (Prodigiosine) hydrochloride,一种天然的红色素,是一种具有生物活性的次生代谢产物。Prodigiosin hydrochloride 是一种有效的促凋亡剂,可抑制Wnt/β-catenin通路。Prodigiosin hydrochloride 具有抗菌,抗真菌,抗原生动物,抗疟疾,免疫抑制和抗癌特性。
生物活性

Prodigiosin (Prodigiosine) hydrochloride is a red pigment produced by bacteria as a bioactive secondary metabolite. Prodigiosin hydrochloride is a potent proapoptotic agent, and inhibitsWnt/β-cateninpathway. Prodigiosin hydrochloride has antibacterial, antifungal, antiprotozoal, antimalarial, immunosuppressive, and anticancer properties[1][2].

体外研究
(In Vitro)

Prodigiosin (25-500 nM; 24 hours) treatment reduces the viability of breast cancer cells, with IC50values at 48 h of 62.52 nM in MDA-MB-231 cells and 261.2 nM in MDA-MB-468 cells[1].
Prodigiosin (25-500 nM; 24 hours) treatment significantly reduces the levels of phosphorylated LRP6 and DVL2, active β-catenin, and total β-catenin. Prodigiosin noticeably inhibits the phosphorylation of GSK3β at Ser9 in HEK293T cells, which is indicative of an increase in GSK3β activity[1].
Prodigiosin can inhibit proliferation and induce apoptosis in breast cancer cells[1].
Prodigiosin (25-500 nM; 24 hours) treatment dose-dependently blocks Wnt signaling activated by Wnt1, Wnt3, Wnt1/LRP6, Wnt3/LRP6, and Dishevelled 2 (DVL2) in transfected HEK293T cells. Prodigiosin treatment inhibits Wnt3A-CM-induced transcription in a dose-dependent manner. Prodigiosin inhibits transcription of the SuperTopFlash reporter activated by either Wnt transfection or Wnt3A treatment[1].
When applied to cultures of chytrid fungiBatrachochytrium dendrobatidisandB. salamandrivorans, Prodigiosin causes significant growth inhibition, with MIC values of 10 μM and 50 μM, respectively[2].

Cell Proliferation Assay[1]

Cell Line:MDA-MB-231 and MDA-MB-468 cells
Concentration:10 nM, 25 nM, 50 nM, 100 nM, 250 nM, 500 nM, 1000 nM, 2500 nM, 5000 nM
Incubation Time:24 hours, 48 hours
Result:Reduced the viability of breast cancer cells, with IC50 values at 48 h of 62.52 nM in MDA-MB-231 cells and 261.2 nM in MDA-MB-468 cells.

Western Blot Analysis[1]

Cell Line:HEK293T cells
Concentration:50 nM, 100 nM, 250 nM, 500 nM
Incubation Time:24 hours
Result:Significantly reduced the levels of phosphorylated LRP6 and DVL2, active β-catenin, and total β-catenin.
体内研究
(In Vivo)

Prodigiosin (5 mg/kg; intraperitoneal injection; twice weekly; for 3 weeks) treatment significantly inhibits tumor growth. Prodigiosin treatment decreases tumor cell density and expression of the proliferation marker Ki-67[1].

Animal Model:Female BALB/c nude mice injected with MDA-MB-231 cells[1]
Dosage:5 mg/kg
Administration:Intraperitoneal injection; twice weekly; for 3 weeks
Result:Significantly inhibited tumor growth in mice.
分子量

359.89

性状

Solid

Formula

C20H26ClN3O

CAS 号

56144-17-3

中文名称

盐酸灵菌红素

结构分类
  • Alkaloids
  • Pyrrole Alkaloids
来源

Serratia marcescens

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)