生物活性
Benpyrine racemate ((Rac)-Benpyrine) is a racemate of Benpyrine, Benpyrine racemate ((Rac)-Benpyrine) is a potent and orally active TNF-α inhibitor and has the potential for TNF-α mediated inflammatory and autoimmune disease research. (Rac)-Benpyrine (5-10 μM; 24 hours) treatment inhibits the expression level of TNF-α downstream pathway protein NF-κB in the nucleus in RAW264.7 macrophages. In vivo,
(Rac)-Benpyrine (25-50 mg/kg/day; oral gavage; 3 weeks) treatment obviously reduces the inflammatory cytokines IFN-γ, IL-1β and IL-6 in the blood serum, and the anti-inflammatory factor IL-10 is obviously increased, the foot swelling degree of the arthritis part is also obviously slowed down.
化学数据
| 分子量 | 308.34 |
| 分子式 | C16H16N6O |
| CAS号 | 1333714-43-4 |
| 纯度 | >99% |
| 溶解性（25°C） | DMSO 9 mg/mL |
| 储存和运输条件 | 2-8°C, protect from light, sealed
常温运输及临时存放 |
不同实验动物依据体表面积的等效剂量转换表（数据来源于FDA指南）
| 小鼠 | 大鼠 | 兔 | 豚鼠 | 仓鼠 | 狗 |
| 重量 (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| 体表面积 (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km系数 | 3 | 6 | 12 | 8 | 5 | 20 |
| 动物 A (mg/kg) = 动物 B (mg/kg) × | 动物 B的Km系数 |
| 动物 A的Km系数 |
例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的Km系数（3），再除以大鼠的Km系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。
储备液配制
以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.2432 mL | 16.2159 mL | 32.4317 mL |
| 5 mM | 0.6486 mL | 3.2432 mL | 6.4863 mL |
| 10 mM | 0.3243 mL | 1.6216 mL | 3.2432 mL |
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