Cell experiment:

MRS broths are supplemented with soy Lecithin concentrations of 0, 0.2, 0.4, 0.6, 0.8 and 1.0%. Each broth is inoculated with a tested strain culture (2%, v/v) and anaerobically incubated at 37℃ for 20 h. After incubation, the bacterium cells are harvested by centrifugation at 8000 g for 10 min at 4℃ and washed twice in PBS (pH 6.5) plus ethanol (5%, v/v). Strain bile resistance is assessed. The numbers of viable cells are counted by the pouring plate method, and each batch is tested three times[1].

Lecithin is regarded as a safe, conventional phospholipid source. Phospholipids are reported to alter the fatty acid composition and microstructure of the membranes in animal cells.

After culturing in MRS broth with 0.2 to 1.0% soy Lecithin, the survival rate of harvested cells increases significantly (P

[1]. Hu B, et al. Enhancement of bile resistance in Lactobacillus plantarum strains by soy lecithin. Lett Appl Microbiol. 2015 Jul;61(1):13-9.