Alphitolic acid (Aophitolic acid) 是一种可以在槲栎植物中提取的抗炎三萜。Alphitolic acid 阻断 Akt-NF-κB 信号传导诱导细胞凋亡(apoptosis)。Alphitolic acid 可以诱导自噬 (autophagy)。Alphitolic acid 具有抗炎活性并下调 NO 和 TNF-α 的产生。Alphitolic acid 可以用作肿瘤和炎症的研究。
| 生物活性 | Alphitolic acid (Aophitolic acid) is an anti-inflammatory triterpene could found in quercus aliena. Alphitolic acid blocks Akt–NF-κB signaling to induceapoptosis. Alphitolic acid inducesautophagy. Alphitolic acid has anti-inflammatory activity and down-regulates the NO and TNF-α production. Alphitolic acid can be used forcancerand inflammation research[1][2][3]. |
体外研究
(In Vitro) | Alphitolic acid (Aophitolic acid) (0-30 μM; 72 hours; HSC-3, SCC2095, and SCC4 cells) has anti-proliferative activity in oral cancer cells in a dose-dependent manner, induces apoptosis and blocks Akt–NF-κB signaling[1].
Alphitolic acid (Aophitolic acid) (0-25 μM; 3-72 hours; SCC4 cells) induces autophagy with increases the expression of autophagosome marker LC3B-II and two autophagyregulatory proteins[1].
Alphitolic acid (Aophitolic acid) (0-25 μM; 72 hours; SCC4 cells) increases p53 phosphorylation and expression, decreases in the expression of the oncogenic E3 ligase MDM2[1].
Alphitolic acid (Aophitolic acid) (0-25 μM; 72 hours; RAW 264.7 macrophages) has anti-inflammatory activity and down-regulates the NO and TNF-α production with IC50values of 17.6 and 22.7 μM, respectively[2].
Cell Viability Assay[1] | Cell Line: | HSC-3, SCC2095 and SCC4 cells | | Concentration: | 0, 10, 15, 20, 25 and 30 μM | | Incubation Time: | 72 hours | | Result: | Suppressed the proliferation of SCC4 and SCC2095 cells with IC50values of 12 and 15 μM, respectively. |
Apoptosis Analysis[1] | Cell Line: | SCC4 cells | | Concentration: | 0, 10, 20 and 30 μM | | Incubation Time: | 72 hours | | Result: | Increased the percentage of apoptotic cells from 11.8% to 25.1% in a dose-dependent manner. |
Western Blot Analysis[1] | Cell Line: | SCC4 cells | | Concentration: | 0, 10, 15 and 20 μM | | Incubation Time: | 72 hours | | Result: | Decreased the expression of phosphorylation of Akt and its downstream substrates, including p70S6K, S6, and IκBα. Down-regulated the expression of NF-κB and its downstream target gene product Bcl-2. |
Western Blot Analysis[1] | Cell Line: | SCC4 cells | | Concentration: | 0, 10, 15, 20 and 25 μM | | Incubation Time: | 3, 6, 12, 24, 48 and 72 hours | | Result: | Increased autophagosome marker LC3B-II in a dose- and time-dependent manner. Increased the expression of autophagy-related protein 7 (Atg7). |
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体内研究
(In Vivo) | Alphitolic acid (Aophitolic acid) (47-756 μg/ear; i.h.; adult male CF-1 mice) has anti-inflammatory activity in vivo[3].
| Animal Model: | Adult male CF-1 mice[3] | | Dosage: | 47, 94, 330, 378 and 756 μg/ear | | Administration: | subcutaneous injection | | Result: | Had anti-inflammatory activity with an ED50of 0.11 and 0.20 μM in a dose-dependent. |
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| 来源 | - Plants
- Myricaceae
- Myrica nagi
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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