Cell lines

BV-2 mouse microglial cells

Preparation method

The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 ℃ for several months.

Reacting condition

0 ~ 1250 μM; 24 hrs

Applications

In BV-2 mouse microglial cells, Ganciclovir inhibited cell proliferation in a dose-dependent manner in the absence of HSVtk, without causing significant cell death at the indicated concentrations. It was implied that Ganciclovir targeted activated microglia at least in part via a mechanism independent of endogenous thymidine kinase (tk).

Animal models

C57BL/6 mice

Dosage form

25 or 100 mg/kg; i.p.

Applications

In C57BL/6 mice, Ganciclovir inhibited the proliferation of microglia induced by experimental autoimmune encephalomyelitis (EAE). Ganciclovir attenuated neuroinflammation in a dose-depedent manner without significantly restraining the peripheral immune response. In addition, it was found that Ganciclovir accumulated in the brains of EAE mice but not those of naive mice to exert localized effects.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

Ganciclovir (DHPG) is a potent antiviral drug used to treatment of cytomegalovirus (CMV) infections [1].

Ganciclovir (DHPG) is a nucleoside analogue and has shown the activity against viral thymidine kinase (tk) in the Herpesviridae family, including human cytomegalovirus and herpes simplex virus types I and II as well as Epstein-Barr virus. In addition, Ganciclovir has been reported to inhibit the proliferation of uninfected cells, most significantly of bone marrow cells, because of the function of inhibiting viral replication. Apart from these, Ganciclovir has been revealed to reduce the activation of microglial in wild-type C57BL/6 mice and inhibit proliferation of microglial in wild-type FvB mice [1].

References:
[1] Ding Z1, Mathur V, Ho PP, James ML, Lucin KM, Hoehne A, Alabsi H, Gambhir SS, Steinman L, Luo J, Wyss-Coray T. Antiviral drug ganciclovir is a potent inhibitor of microglial proliferation and neuroinflammation. J Exp Med. 2014 Feb 10; 211(2):189-98.