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Naph-EA-mal
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Naph-EA-mal图片
CAS NO:210292-65-2
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
50mg电议
100mg电议

产品名称
Thiol-green 1
产品介绍
Naph-EA-mal (Thiol-green 1) 是一种快速检测和超快 turn-on 型硫醇 (thiol) 荧光探针,可用于蛋白标记和生物成像。Naph-EA-mal (Thiol-green 1) 可用于检测活细胞中的硫醇,标记蛋白质硫醇,定量细胞裂解液中总硫醇的浓度,并确定固定细胞中可逆的蛋白质硫醇氧化。Ex: 488 nM; Em: 540 nM。
生物活性

Naph-EA-mal (Thiol-green 1) is a rapid detect and ultrafast turn-onthiol fluorescence probefor protein labeling and bioimaging. Naph-EA-mal (Thiol-green 1) can be used to detect thiols in living cells, label the protein thiols, quantify the concentration of total thiols in cell lysate, and determine the reversible protein thiols oxidation in fixed cells[1]. Ex: 488 nM; Em: 540 nM.

体外研究
(In Vitro)

Imaging thiols in living cells[1]
Hep G2 cells incubated with Naph-EA-mal (Thiol-green 1) (1 μM;5 mins), and strong green fluorescence can be observed. When cells are preincubated the cells to block the intracellular thiols with NEM (a thiol block reagent; 100 μM), a weaker fluorescence is observed in cells[1].
Monitoring thiol-disulfide levels in fixed cells
Due to the majority of cellular thiols are present in the reduced form, Naph-EA-mal (Thiol-green 1) (10 μM) induces strong fluorescence in Hep G2 cells. After diamide treatment, fluorescent signal sharp dimed is observed[1].
The Hep G2 cells, oxidized by diamide (200 μM; 30 min), are fixed by 70% EtOH and the remaining free thiols are blocked by NEM (100 μM). then The cells are further treated with TCEP (5 mM) followed by addition of the Naph-EA-mal (Thiol-green 1) (10 μM). The green fluorescent signal is detected again[1]

分子量

391.42

性状

Solid

Formula

C22H21N3O4

CAS 号

210292-65-2

Emission (Em)
Em 495-570 nm Green
Excitation (Ex)
Ex 450-495 nm Blue
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 33.33 mg/mL(85.15 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.5548 mL12.7740 mL25.5480 mL
5 mM0.5110 mL2.5548 mL5.1096 mL
10 mM0.2555 mL1.2774 mL2.5548 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

染色示例
  • Description: Naph-EA-mal is a thiol fluorescence probe, it can be used to label the protein thiols.
    Method: For measurement of total thiol level in bacteria.
    1. Incubate bacteria with Naph-EA-mal (10 min; room temperature; dark).
    2. 106CFU/mL bacteria are transferred to slide for analysis.
    3. The images are captured using 100×oil immersion and observe under the confocal microscope.