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Spectinomycin dihydrochloride
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Spectinomycin dihydrochloride图片
CAS NO:21736-83-4
包装与价格:
包装价格(元)
10 mM * 1 mL in Water电议
500mg电议
1 g电议
5 g电议
25 g电议
50 g电议

产品名称
盐酸奇霉素
产品介绍
Spectinomycin dihydrochloride 是一种广谱抗生素,可以抑制多种革兰氏阳性和革兰氏阴性细菌的生长。Spectinomycin dihydrochloride 通过特异靶向细菌核糖体,干扰蛋白质合成。Spectinomycin dihydrochloride 也是 td 内含子 RNA 的非竞争性抑制剂,Ki为 7.2 mM。
生物活性

Spectinomycin dihydrochloride is a broad-spectrumantibioticand inhibits the growth of a variety of gram-positive and gram-negative organisms. Spectinomycin dihydrochloride acts by selectively targeting to thebacterialribosome and interrupting protein synthesis. Spectinomycin dihydrochloride is also a noncompetitive inhibitor of td intron RNA with anKivalue of 7.2 mM[1]-[5].

IC50& Target

bacterial ribosomal subunit[5]

体外研究
(In Vitro)

Spectinomycin dihydrochloride selectively inhibits protein synthesis in cells and in extracts ofEscherichia coli:
Spectinomycin dihydrochloride (50 μg/mL) inhibitsEscherichia coligrowth rapidly and reversibly, and suppresses amino acid incorporation immediately[1].
Spectinomycin dihydrochloride (1 μg/mL or 3 μM) inhibits polypeptide synthesis directed either by endogenous messenger RNA or by MS-2 bacteriophage RNA, with maximum inhibition of 70-80% in extracts ofEscherichia coli[1][1].
Spectinomycin dihydrochloride blocks the translocation of peptidyl-tRNAs from A-site to P-site by inhibiting the binding of elongation factor G to the ribosome[2].
Spectinomycin dihydrochloride interacts specifically with the residues G1064 and 01192 in 16S rRNA and potentially makes it inactive[2].
Spectinomycin dihydrochloride exhibits splicing inhibition and dependent on pH changes and Mg2+concentration, indicating electrostatic interactions with the intron RNA[3].

体内研究
(In Vivo)

Spectinomycin dihydrochloride (20 mg/kg; i.m.; 20-100 mg/kg; 9 d) shows the safety in healthy chicks[4].
Spectinomycin dihydrochloride (10 mg/kg; i.v.; single dose) has the major elimination pathway by renal excretion, approximately 55% is excreted into the urine in unchanged form[5].
Pharmacokinetics of Spectinomycin dihydrochloride in Rat[5]

ParameterC0(μg/mL)AUC0-∞(μgoh/mL)Vd(L/kg)CL (L/h/kg)MRT (h)T1/2α(h)T1/2β(h)T1/2γ(h)feCLrenal(L/h/kg)Eratio
Non atrioventricular analysis44.316.80.7560.6020.757///0.5530.3591.00
Three-compartment model37.815.70.7470.6491.11/0.2370.75419.5//

Animal Model:Arbor Acres plus broiler chicks (15-day-old)[4]
Dosage:20 mg/kg, 60 mg/kg, 100 mg/kg
Administration:Intramuscular injection (chest muscles); 9 days
Result:Showed biosecurity of 20 mg/kg by complete blood count, biochemical parameters, histopathological, clinical signs, body weight gain, and feed conversion ratio (FCR).
Resulted minor toxicity of 60 mg/kg.
分子量

405.27

性状

Solid

Formula

C14H26Cl2N2O7

CAS 号

21736-83-4

中文名称

盐酸奇霉素;盐酸壮观霉素

结构分类
  • Antibiotics
  • Other Antibiotics
来源

bacterium Streptomyces spectabilis

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : ≥ 100 mg/mL(246.75 mM)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.4675 mL12.3375 mL24.6749 mL
5 mM0.4935 mL2.4675 mL4.9350 mL
10 mM0.2467 mL1.2337 mL2.4675 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 50 mg/mL (123.37 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。