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DENV-IN-4
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
DENV-IN-4图片
CAS NO:2762302-75-8
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品介绍
DENV-IN-4 是一种有效的DENV抑制剂 (DENV EC50=4.79 μM, Vero CC50>100 μM, SI>20.9)。DENV-IN-4 可抑制 DENV2 的表达水平,具有浓度依赖性,并且能降低依赖性 RNA 聚合酶 (RNA-dependent RNA polymerase, RdRp) 的活性。DENV-IN-4 具有抗病毒作用。
生物活性

DENV-IN-4 is a potentDENVinhibitor (DENV EC50=4.79 μM, Vero CC50>100 μM, SI>20.9). DENV-IN-4 can inhibit the expression level of DENV2 with concentration-dependence and reduce RNA-dependent RNA polymerase (RdRp) enzymatic activity. DENV-IN-4 has antiviral effect[1].

IC50& Target

EC50=4.79 μM (DENV)[1]

体外研究
(In Vitro)

DENV-IN-4 (compound 15) (0.064-200 μM; 3 days) has antiviral effect and not related to its toxicity (DENV EC50=4.79 μM, Vero CC50>100, SI>20.9)[1].
DENV-IN-4 (3.125-50 μM in Vero, 3.75-30 μM in A549 and Huh-7; 48 hours) inhibits the expression level of DENV2 with concentration-dependence, and inhibition curves were smoother in Vero[1].
DENV-IN-4 (7.5 and 15 μM; 48 hours) can effectively inhibit DENV infection in Vero cells, and completely inhibited at 15 μM[1].
DENV-IN-4 (0-15 μM; 48 hours) inhibits DENV2 E protein production with good concentration-dependent relationship in Vero cells[1].

Cell Cytotoxicity Assay

Cell Line:Vero[1]
Concentration:0.064-200 μM
Incubation Time:3 days
Result:Showed antiviral effect of DENV-IN-4 was not related to its toxicity (Vero EC50=4.79 μM, CC50>100, SI>20.9).

RT-PCR

Cell Line:Vero, A549 and Huh-7 cells[1]
Concentration:3.125-50 μM in Vero, 3.75-30 μM in A549 and Huh-7
Incubation Time:48 hours
Result:Inhibited the expression level of DENV2 with concentration-dependence, and inhibition curves were smoother in Vero.

Western Blot Analysis

Cell Line:Vero[1]
Concentration:7.5 and 15 μM
Incubation Time:48 hours
Result:Effectively inhibited DENV infection and completely inhibited at 15 μM.

Immunofluorescence

Cell Line:Vero[1]
Concentration:0-15 μM
Incubation Time:48 hours
Result:Inhibited DENV2 E protein production with good concentration-dependent relationship.
分子量

516.66

Formula

C28H32N4O4Si

CAS 号

2762302-75-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.