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PTD-p65-P1 Peptide TFA
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
包装与价格:
包装价格(元)
1mg电议
5mg电议
10mg电议
50mg电议
100mg电议

产品介绍
PTD-p65-P1 Peptide TFA 是一种有效的选择性核转录因子NF-κB抑制剂,来源于 NF-κB 氨基酸残基 271-282 的 p65 亚单位,可选择性抑制各种炎症刺激诱导的 NF-κB 激活,下调 NF-κB 介导的基因表达,并上调细胞凋亡(apoptosis)。
生物活性

PTD-p65-P1 Peptide TFA is a potent, selective nuclear transcription factorNF-κBinhibitor and derives from the p65 subunit ofNF-κBamino acid residues 271-282, which selectively inhibitsNF-κBactivation induced by various inflammatory stimulation, down-regulate NF-κB-mediated gene expression and up-regulateapoptosis[1][2].

IC50& Target

NF-kappaB[1]

体外研究
(In Vitro)

PTD-p65-P1 Peptide TFA (10-150 μM; 0-60 min; KBM-5 cells) inhibits TNF-induced NF-κB activation in a dose-dependent manner and suppresses TNF-induced NF-κB activation by 25% at 100 μM and completely at 150 μM[1].
PTD-p65-P1 Peptide TFA (150 μM; 0-60 min; KBM-5 cells) inhibits cytoplasmic p65 phosphorylation and nuclear translocation[1].
PTD-p65-P1 Peptide TFA (100 μM; 16 h; KBM-5 cells) potentiates the TNF-induced apoptosis from 4 to 45%[1].
PTD-p65-P1 Peptide TFA (150 μM; A 293 cells) inhibits NF-κB-dependent reporter gene expression induced by TNF[1].
PTD-p65-P1 Peptide TFA (150 μM; 12-24 h; SP-53 cells ) inhibits the cell proliferation[2].

Western Blot Analysis[1]

Cell Line:KBM-5 cells
Concentration:150 μM
Incubation Time:0, 5, 10, 15, 30 and 60 minutes
Result:Suppressed TNF-induced NF-κB activation by inhibiting phosphorylation and nuclear translocation of p65.

Cell Proliferation Assay[2]

Cell Line:SP-53 cells
Concentration:150 μM
Incubation Time:12 and 24 hours
Result:Inhibited cell proliferation of 40% in 12 hours and 60% in 24 hours.
分子量

3943.52

性状

Solid

Formula

C170H276F3N57O46S

Sequence Shortening

DRQIKIWFQNRRMKWKKQLRRPSDRELSE

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light, under nitrogen

Powder-80°C2 years
-20°C1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)