Quinoclamine 是一种萘醌衍生物，是一种NF-κB抑制剂。Quinoclamine 具有抗肿瘤活性。
| 生物活性 | Quinoclamine, a naphthoquinone derivative, is aNF-κBinhibitor. Quinoclamine exhibits anti-cancer activity[1][2]. |
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体外研究
(In Vitro) | Quinoclamine causes differentiation of U-937 cells into macrophage-like cells[1].
Quinoclamine inhibits NF-κB activities in HepG2 cells, with an IC50of 1.7 μM[2].
Quinoclamine (1-4 μM; 30 minutes ) suppresses endogenous NF-κB activity in HepG2 cells through the inhibition of IκB-α phosphorylation and p65 translocation[2].
Quinoclamine inhibits induced NF-κB activities in lung and breast cancer cell lines[2].
Quinoclamine affects the expression levels of genes involved in cell cycle or apoptosis[2].
Quinoclamine down-regulates the expressions of UDP glucuronosyltransferase genes involved in phase II drug metabolism[2].
Cell Viability Assay[2] | Cell Line: | HepG2 cells | | Concentration: | 1 μM, 2 μM, 4 μM, 8 μM, 16 μM, 32 μM, 64 μM | | Incubation Time: | 24 hours | | Result: | Inhibited NF-κB activities in HepG2 cells. |
Western Blot Analysis[2] | Cell Line: | HepG2 cells | | Concentration: | 0 μM, 1 μM, 2 μM, 4 μM | | Incubation Time: | 30 minutes | | Result: | Inhibited IκB-α phosphorylation and p65 translocation in HepG2 cells. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : 250 mg/mL(1204.18 mM;Need ultrasonic) 配制储备液 | 1 mM | 4.8167 mL | 24.0836 mL | 48.1672 mL | | 5 mM | 0.9633 mL | 4.8167 mL | 9.6334 mL | | 10 mM | 0.4817 mL | 2.4084 mL | 4.8167 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: 2.08 mg/mL (10.02 mM); Clear solution; Need ultrasonic
此方案可获得 2.08 mg/mL (10.02 mM) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 *以上所有助溶剂都可在本网站选购。
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