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IKKγNBD Inhibitory Peptide
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
CAS NO:372146-18-4
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品介绍
IKKγ NBD 抑制肽是一种高度特异性的NF-κB抑制剂。IKKγ NBD 抑制肽通过阻断 IKKγ/NEMO 结合域 (NBD) 与 IKKα 和 IKKβ 之间的相互作用,而阻断 TNF-α 诱导的NF-κB激活。IKKγ NBD 抑制肽可显著降低炎症、改善脑缺血所致的神经功能缺损。
生物活性

IKKγ NBD Inhibitory Peptide is a highly specific inhibitorNF-κBinhibitor. IKKγ NBD Inhibitory Peptide acts by disrupting the interaction between IKKγ/NEMO-binding domain (NBD) with IKKα and IKKβ, thus blocking TNF-α-induced NF-kB activation. IKKγ NBD Inhibitory Peptide could significantly suppresses inflammation and ameliorate the cerebral ischemia-induced neurological deficits[1][2][3].

体外研究
(In Vitro)

在一个典型途径中,NF-κB 的激活依赖于 IKK 复合体的活性,该复合体由三个亚基 (IKKκ、IKKβ 和 IKKγ/NEMO) 组成,因此,KKγ NBD 抑制肽抑制 TNF-α 诱导的 NF- kb激活[2]
IKKγ NBD 抑制肽 (10 μM; 90 min) 能够阻碍Doxorubicin(HY-15142A) 诱导 (15 μM;4 h) 的 BT-474 细胞中 p65 磷酸化[2]
IKKγ NBD 抑制肽 (10 μM; 48 h) 对BT-474细胞活力有轻微抑制作用,而对 Doxorubicin 的效力有增强作用[2]

体内研究
(In Vivo)

IKKγ NBD 抑制肽 (40 μg/4 μL;ICV; 单剂量; 于 MCAO 诱导前 2 h) 在大鼠模型脑中动脉闭塞 (MCAO) 模型中显示出神经保护作用,减少 DNA 碎片化,其机制可能与减少缺血损伤后的炎症有关[3]

Animal Model:Adult male Sprague-Dawley rats (255 g) with middle cerebral artery occlusion (MCAO)[3]
Dosage:40 μg/4 μL
Administration:Intracerebroventricular injection; 2 h before MCAO induction
Result:Significantly reduced striatal IL-1b level in IKK-NBD peptide treated rats.
Also resulted in reduced staining of microglial OX-42 and protection of BBB from ischemic insult.
分子量

3693.30

Formula

C170H259N49O42S1

CAS 号

372146-18-4

Sequence Shortening

DRQIKIWFQNRRMKWKKTALDWSWLQTE

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

 ContentsAssign value
Acidic amino acidAsp (D), Glu (E), and the C-terminal -COOH.-1
Basic amino acidArg (R), Lys (K), His (H), and the N-terminal -NH2+1
Neutral amino acidGly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)0

3.  Recommended solution:

Overall charge of peptideDetails
Negative (<0)1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.