Eurycomalactone

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Eurycomalactone

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

23062-24-0

包装与价格：

包装	价格(元)
1mg	电议
5mg	电议
10mg	电议
50mg	电议

产品名称

东革内酯

产品介绍

Eurycomalactone 是一种可以从Eurycoma longifolia Jack中分离得到的活性拟松类化合物。Eurycomalactone 是一种有效的NF-κB抑制剂，其IC₅₀值为 0.5 μM。Eurycomalactone 可抑制蛋白合成，降低 cyclin D1 蛋白水平。Eurycomalactone 通过阻滞细胞周期于G2/M 期和延迟 DNA 双链断裂修复来提高放射敏感性。Eurycomalactone 抑制 AKT/NF-κB 信号通路的激活，诱导细胞凋亡并增强对 Cisplatin (HY-17394) 的化疗敏感性。

生物活性

Eurycomalactone is an active quassinoid could be isolated fromEurycoma longifolia Jack. Eurycomalactone is a potentNF-κBinhibitor with anIC₅₀value of 0.5 μM. Eurycomalactone inhibits protein synthesis and depletes cyclin D1. Eurycomalactone enhances radiosensitivity through arrest cell cycle at G2/M phase and delayed DNA double-strand break repair. Eurycomalactone inhibits the activation of AKT/NF-κB signaling, inducesapoptosisand enhances chemosensitivity toCisplatin(HY-17394)^[1]^[2]^[3].

IC₅₀& Target

IC50: 0.5 μM (NF-κB)^[1]

体外研究
(In Vitro)

Eurycomalactone (24, 48 and 72 h) selectively inhibits the viability of A549 and COR-L23 cells Eurycomalactone inhibits the viability of A549 cells with IC₅₀values of 20.17, 3.77, and 1.90 μM for 24, 48 and 72 hours, respectively. Eurycomalactone inhibits the viability of COR-L23 cells with IC₅₀values of 25.02, 2.74, and 1.80 μM for 24, 48 and 72 hours, respectively^[1].
Eurycomalactone (2.29-156.3 μM; 24 h; A549 and Calu-1 cells) promotes Non-small cell lung cancer (NSCLC) cells apoptosis^[2].
Eurycomalactone (0-25.05 μM; 24 h; A549 and COR-L23 cells) induces cell cycle arrest at the radiosensitive G2/M phase and induces apoptosis in irradiated Non-small cell lung cancer (NSCLC) cells. Eurycomalactone downregulated the key G2/M regulatory proteins in irradiated Non-small cell lung cancer (NSCLC) cells^[1].
Eurycomalactone (2.5-25 μM; 24 h; A549 cells) suppressed the repair of radiation-Induced DNA double-strand breaks^[1].
Eurycomalactone (2.29-156.3 μM; 24 h; A549 and Calu-1 cells) suppresses AKT/NF-κB activation in Non-small cell lung cancer (NSCLC) cells^[2].

Apoptosis Analysis^[2]

Cell Line:	A549 and Calu-1 cells
Concentration:	2.29, 10.14, 12.02, 20.81, 80.77, and 156.3 μM
Incubation Time:	24 hours
Result:	Increased the apoptotic rates in a dose-dependent manner.

Cell Cycle Analysis^[1]

Cell Line:	A549 and COR-L23 cells
Concentration:	1.57, 2.57, 20.17 and 25.05 μM
Incubation Time:	24 hours
Result:	Induced cell cycle arrest at G2/M phase in irradiated cells and increased the sub-G1 population. A549 and COR-L23 cells

Western Blot Analysis^[2]

Cell Line:	A549 and Calu-1 cells
Concentration:	2.29, 10.14, 12.02, 20.81, 80.77, and 156.3 μM
Incubation Time:	24 hours
Result:	Induced the expression levels of active caspase-3 and active PARP (cleaved form), while decreased Bcl-xL and surviving.

Western Blot Analysis^[1]

Cell Line:	A549 and COR-L23 cells
Concentration:	1.57, 2.57, 20.17 and 25.05 μM
Incubation Time:	24 hours
Result:	Downregulated the expression of both G2/M regulatory proteins in a dose-dependent manner.

Western Blot Analysis^[2]

Cell Line:	A549 and COR-L23 cells
Concentration:	2.29, 10.14, 12.02, 20.81, 80.77, and 156.3 μM
Incubation Time:	24 hours
Result:	inhibited the expression levels of p^(S473)-AKT, total AKT, p^(S536)-NF-κB p65 and total NF-κB p65.

分子量

348.39

性状

Solid

Formula

C₁₉H₂₄O₆

CAS 号

23062-24-0

中文名称

东革内酯

结构分类

Terpenoids
Diterpenoids

来源

Plants
other families

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 100 mg/mL(287.03 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	2.8703 mL	14.3517 mL	28.7035 mL
5 mM	0.5741 mL	2.8703 mL	5.7407 mL
10 mM	0.2870 mL	1.4352 mL	2.8703 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。