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Handelin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Handelin图片
CAS NO:62687-22-3
包装与价格:
包装价格(元)
5mg电议
10mg电议
50mg电议
100mg电议

产品介绍
Handelin 是来自Chrysanthemum boreale的愈创木酚内酯二聚体,它通过下调NF-κB信号传导和促炎性细胞因子的产生而具有强大的抗炎活性。
生物活性

Handelin is a guaianolide dimer fromChrysanthemum borealethat has potent anti-inflammatory activity by down-regulatingNF-κBsignaling and pro-inflammatory cytokine production[1].

体外研究
(In Vitro)

Handelin (Compound 1; 10-40 μM; RAW 264.7 cells) treatment suppresses the LPS-induced (1 μg/mL) overexpression of iNOS and COX-2 protein levels in a concentration-dependent manner. Handelin also suppresseS the induction of pro-inflammatory cytokines TNF-α and IL-1β in LPS-stimulated RAW 264.7 cells. Handelin also suppresses the activation of mitogen-activated protein kinases, including ERK and JNK signaling[1].
Handelin (Compound 1; 10-40 μM; RAW 264.7 cells) treatment significantly reduces the iNOS and COX-2 mRNA levels in LPS- stimulated RAW 264.7 cells. The transcriptional activity of NF-κB stimulated with LPS is also suppressed by Handelin. In addition, the LPS-stimulated upregulation of miRNA-155 expression is suppressed by Handelin[1].

RT-PCR[1]

Cell Line:RAW 264.7 cells
Concentration:10 μM, 20 μM, 40 μM
Incubation Time:5 hours
Result:iNOS and COX-2 mRNA levels were significantly reduced. The transcriptional activity of NF-κB stimulated with LPS was also suppressed and the LPS-stimulated upregulation of miRNA-155 expression was also suppressed.

Western Blot Analysis[1]

Cell Line:RAW 264.7 cells
Concentration:10 μM, 20 μM, 40 μM
Incubation Time:
Result:Suppressed the LPS-induced overexpression of iNOS and COX-2 protein levels in a concentration-dependent manner. Suppressed the induction of pro-inflammatory cytokines TNF-α and IL-1β, and also also suppressed the activation of mitogen-activated protein kinases, including ERK and JNK signaling in LPS-stimulated RAW 264.7 cells.
体内研究
(In Vivo)

Handelin (Compound 1; 10-20 mg/kg; oral administration; for 30 mintues; male Sprague-Dawley rats) treatment inhibits acute inflammation in carrageenan-induced paw edema model. The serum level of IL-1β is also inhibited by Handelin in a carrageenan-induced paw edema model[1].

Animal Model:Male Sprague-Dawley (SD) rats (150-170 g, 5 weeks old) injected with carrageenan[1]
Dosage:10 mg/kg, 20 mg/kg
Administration:Oral administration; for 30 mintues
Result:Inhibited acute inflammation in carrageenan-induced paw. The serum level of IL-1β was also inhibited.
分子量

552.66

性状

Solid

Formula

C32H40O8

CAS 号

62687-22-3

结构分类
  • Terpenoids
  • Sesquiterpenes
来源
  • Plants
  • Compositae
  • Chrysanthemum boreale
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO : 16.67 mg/mL(30.16 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.8094 mL9.0472 mL18.0943 mL
5 mM0.3619 mL1.8094 mL3.6189 mL
10 mM0.1809 mL0.9047 mL1.8094 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: 1.67 mg/mL (3.02 mM); Suspended solution; Need ultrasonic

    此方案可获得 1.67 mg/mL (3.02 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: 1.67 mg/mL (3.02 mM); Suspended solution; Need ultrasonic

    此方案可获得 1.67 mg/mL (3.02 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 1.67 mg/mL (3.02 mM); Clear solution

    此方案可获得 ≥ 1.67 mg/mL (3.02 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。