FW1256

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FW1256

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

117089-08-4

包装与价格：

包装	价格(元)
10 mM * 1 mL in DMSO	电议
5mg	电议
10mg	电议
50mg	电议
100mg	电议
200mg	电议

产品介绍

FW1256 是一种苯基类似物，也是一种缓释硫化氢 (H₂S) 供体。FW1256 抑制NF-κB活性，并可诱导细胞凋亡 (apoptosis)，具有有效的抗炎作用，并可用于癌症和心血管疾病的研究。

生物活性

FW1256 is a phenyl analogue and a slow-releasing hydrogen sulfide (H₂S) donor. FW1256 inhibitsNF-κBactivity and induces cellapoptosis. FW1256 exerts potent anti-inflammatory effects and has the potential forcancerandcardiovascular diseasetreatment^[1]^[2].

体外研究
(In Vitro)

FW1256 (200 μM; 24.5 hours; AW264.7 cells) treatment significantly reduces IL-1β, COX-2 and iNOS mRNA and protein in LPS-stimulated RAW264.7 macrophages^[1].
FW1256 (200 μM; 24.5 hours; AW264.7 cells) treatment significantly reduces IL-1β, COX-2 and iNOS PROTE and protein in LPS-stimulated RAW264.7 macrophages^[1].
FW1256 concentration dependently decreases TNF-α (IC₅₀of 61.2 μM), IL-6 (IC₅₀of 11.7 μM), PGE2 (IC₅₀of 25.5 μM) and NO (IC₅₀of 34.6 μM) generation in LPS-stimulated RAW264.7 macrophages and bone marrow-derived macrophages (BMDMs) (IC₅₀s of 414.9 μM, 300.2 μM, 4 μM and 9.5 μM for TNF-α, IL-6, PGE2 and NO, respectively)^[1].
FW1256 decreases NF-κB activation as evidenced by reduced cytosolic phospho-IκBα levels and reduces nuclear p65 levels in LPS-stimulated RAW264.7 macrophages treated with FW1256^[1].

RT-PCR^[1]

Cell Line:	RAW264.7 cells
Concentration:	200 μM
Incubation Time:	24.5 hours
Result:	Significantly reduced IL-1β, COX-2 and iNOS mRNA in LPS-stimulated RAW264.7 macrophages..

Western Blot Analysis^[1]

Cell Line:	RAW264.7 cells
Concentration:	200 μM
Incubation Time:	24.5 hours
Result:	Significantly reduced IL-1β, COX-2 and iNOS proteinin LPS-stimulated RAW264.7 macrophages..

体内研究
(In Vivo)

FW1256 (100 mg/kg; intraperitoneal injection; male C57BL/6 mice) treatment reduces IL-1β, TNFα, nitrate/nitrite and PGE2 levels in LPS-treated mice^[1].

Animal Model:	Male C57BL/6 mice (20-25 g, 6-10 weeks) injected withE. colilipopolysaccharide (LPS)^[1]
Dosage:	100 mg/kg
Administration:	Intraperitoneal injection
Result:	Reduced IL-1β, TNFα, nitrate/nitrite and PGE2 levels in LPS-treated mice.

分子量

247.25

性状

Solid

Formula

C₁₂H₁₀NOPS

CAS 号

117089-08-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : ≥ 250 mg/mL(1011.12 mM)

*"≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	4.0445 mL	20.2224 mL	40.4449 mL
5 mM	0.8089 mL	4.0445 mL	8.0890 mL
10 mM	0.4044 mL	2.0222 mL	4.0445 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。