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FW1256
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
FW1256图片
CAS NO:117089-08-4
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
50mg电议
100mg电议
200mg电议

产品介绍
FW1256 是一种苯基类似物,也是一种缓释硫化氢 (H2S) 供体。FW1256 抑制NF-κB活性,并可诱导细胞凋亡 (apoptosis),具有有效的抗炎作用,并可用于癌症和心血管疾病的研究。
生物活性

FW1256 is a phenyl analogue and a slow-releasing hydrogen sulfide (H2S) donor. FW1256 inhibitsNF-κBactivity and induces cellapoptosis. FW1256 exerts potent anti-inflammatory effects and has the potential forcancerandcardiovascular diseasetreatment[1][2].

体外研究
(In Vitro)

FW1256 (200 μM; 24.5 hours; AW264.7 cells) treatment significantly reduces IL-1β, COX-2 and iNOS mRNA and protein in LPS-stimulated RAW264.7 macrophages[1].
FW1256 (200 μM; 24.5 hours; AW264.7 cells) treatment significantly reduces IL-1β, COX-2 and iNOS PROTE and protein in LPS-stimulated RAW264.7 macrophages[1].
FW1256 concentration dependently decreases TNF-α (IC50of 61.2 μM), IL-6 (IC50of 11.7 μM), PGE2 (IC50of 25.5 μM) and NO (IC50of 34.6 μM) generation in LPS-stimulated RAW264.7 macrophages and bone marrow-derived macrophages (BMDMs) (IC50s of 414.9 μM, 300.2 μM, 4 μM and 9.5 μM for TNF-α, IL-6, PGE2 and NO, respectively)[1].
FW1256 decreases NF-κB activation as evidenced by reduced cytosolic phospho-IκBα levels and reduces nuclear p65 levels in LPS-stimulated RAW264.7 macrophages treated with FW1256[1].

RT-PCR[1]

Cell Line:RAW264.7 cells
Concentration:200 μM
Incubation Time:24.5 hours
Result:Significantly reduced IL-1β, COX-2 and iNOS mRNA in LPS-stimulated RAW264.7 macrophages..

Western Blot Analysis[1]

Cell Line:RAW264.7 cells
Concentration:200 μM
Incubation Time:24.5 hours
Result:Significantly reduced IL-1β, COX-2 and iNOS proteinin LPS-stimulated RAW264.7 macrophages..
体内研究
(In Vivo)

FW1256 (100 mg/kg; intraperitoneal injection; male C57BL/6 mice) treatment reduces IL-1β, TNFα, nitrate/nitrite and PGE2 levels in LPS-treated mice[1].

Animal Model:Male C57BL/6 mice (20-25 g, 6-10 weeks) injected withE. colilipopolysaccharide (LPS)[1]
Dosage:100 mg/kg
Administration:Intraperitoneal injection
Result:Reduced IL-1β, TNFα, nitrate/nitrite and PGE2 levels in LPS-treated mice.
分子量

247.25

性状

Solid

Formula

C12H10NOPS

CAS 号

117089-08-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : ≥ 250 mg/mL(1011.12 mM)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM4.0445 mL20.2224 mL40.4449 mL
5 mM0.8089 mL4.0445 mL8.0890 mL
10 mM0.4044 mL2.0222 mL4.0445 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。