(-)-Hinesol (Hinesol) 是一种有效的抗癌剂。(-)-Hinesol 诱导细胞凋亡 (apoptosis) 和细胞周期停滞在 G0/G1 期 (-)-Hinesol 下调 MEK/ERK 通路和 NF-κB 通路并介导 cyclin D1、Bax 和 Bcl-2 的表达。(-)-Hinesol 具有研究非小细胞肺癌的潜力。
| 生物活性 | (-)-Hinesol (Hinesol) is a potentanticanceragent. (-)-Hinesol inducesapoptosisand cell cycle arrest at G0/G1 phase. (-)-Hinesol downregulates MEK/ERK pathway andNF-κBpathway and mediates theexpression of cyclin D1,BaxandBcl-2. (-)-Hinesol has the potential for the research of non–small cell lungcancer[1]. |
体外研究
(In Vitro) | (-)-Hinesol (0-25 μg/ml; 24, 48 h) shows antiproliferative activity of the A549 and NCI-H1299 cells in a dose- and time-dependent manner[1].
(-)-Hinesol (0, 2, 8 μg/ml; 24 h) induces apoptosis and cell cycle arrest at G0/G1 phase with increases the expression of Bax and decreases the expression of Bcl-2 and cyclin D1[1].
(-)-Hinesol (0, 2, 8 μg/ml; 24 h) decreases the expression of phosphor-ERK1/2, phosphor-MEK1/2, phosphor-IκBα and -p65 level, and shows no change for the total protein levels of IκBα and p65 in A549 cells[1].
Cell Proliferation Assay[1] | Cell Line: | A549, NCI-H1299 cells | | Concentration: | 0-25 μg/ml | | Incubation Time: | 24, 48 h | | Result: | Inhibited the proliferation of the A549 and NCI-H1299 cells in a dose- and time-dependent manner. |
Apoptosis Analysis[1] | Cell Line: | A549 cells | | Concentration: | 0, 2, 8 μg/ml | | Incubation Time: | 24 h | | Result: | Induced apoptosis with the apoptotic cells was increased to 21.2 ± 0.96% and 36 ± 1.04% after treatment with hinesol at 2 and 8 μg/mL, respectively. |
Western Blot Analysis[1] | Cell Line: | A549 cells | | Concentration: | 0, 2, 8 μg/ml | | Incubation Time: | 24 h | | Result: | Increased the protein expression of Bax and decreased the expression of Bcl-2. |
Cell Cycle Analysis[1] | Cell Line: | A549 cells | | Concentration: | 0, 2, 8 μg/ml | | Incubation Time: | 24 h | | Result: | Showed concentration-dependent increase in the percentage of cell in the G0/G1 phase, and a decrease of the percentage in G2/M phase. |
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| 来源 | - Plants
- Leguminosae
- Erythrina sigmoideaHua
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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