Clenbuterol (NAB-365) 是一种β2-肾上腺素能受体激动剂，EC50为 31.9 nM。Clenbuterol 有效抑制脂多糖 (LPS) 诱导的 TNF-α 和 IL-1β 释放。Clenbuterol 可以抑制炎症过程。Clenbuterol 可用作支气管扩张剂。
| 生物活性 | Clenbuterol (NAB-365) is aβ2-adrenergic receptoragonist with anEC50of 31.9 nM[1]. Clenbuterol is a very potent inhibitor of the lipopolysaccharide (LPS)-induced release of TNF-α and IL-1β. Clenbuterol can inhibit the inflammatory process. Clenbuterol is a bronchodilator[2]. |
体外研究
(In Vitro) | Clenbuterol (NAB-365) is a selective β2-adrenergic agonist (β2/β1 ratio = 4.0). Clenbuterol is a very potent inhibitor of the lipopolysaccharide (LPS)-induced release of TNF-α and IL-1β. Clenbuterol can inhibit the inflammatory process[2].
Clenbuterol (10-200 μM; for 24 or 48 hours) decreases the viability of C2C12 myoblasts. Clenbuterol (100 μM) significantly decreases DNA synthesis. Clenbuterol (100 μM; for 12 h) increases the proportion of cells in G0/G1 phase. Clenbuterol treatment delays cell cycle progression. Clenbuterol (100 μM) induces cell cycle arrest, but not apoptosis, in C2C12 myoblasts[3].
Cell Viability Assay[3] | Cell Line: | C2C12 cells. | | Concentration: | 0, 10, 100, and 200 μM. | | Incubation Time: | 24 and 48 hours. | | Result: | Treatment reduced viability of C2C12 cells for 24 and 48 h. |
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体内研究
(In Vivo) | Treatment with Clenbuterol increases survival, rescues abnormalities in respiratory function and social recognition, and improves motor coordination in young maleMecp2-null (Mecp2–/y) mice[4].
Clenbuterol is a bronchodilator. Clenbuterol (90 μg) is administered intratracheally to five horses. Peak serum concentrations of ~230 pg/mL are detected 10 min after administration, dropping to ~50 pg/mL within 30 min and declining much more slowly thereafter. Intratracheal administration of clenbuterol shortly before race time can be detected with this serum test[5].
| Animal Model: | MaleMecp2–/yand femaleMecp2–/+mice[4] | | Dosage: | 5 or 0.1 mg/kg. | | Administration: | Injected i.p.; daily for 5 d, followed by a 2-d off period, repeated weekly | | Result: | Significantly improved the phenotype during the second but not first day of testing, implying a modest effect on motor coordination in Mecp2-null mice. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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