Protocatechualdehyde (Catechaldehyde) 是从Salvia miltiorrhiza中分离出来的一种天然多酚化合物,具有多种生物活性,可作为抗氧化、抗衰老、抗菌消炎剂被广泛应用于多个医学领域。
生物活性 | Protocatechualdehyde (Catechaldehyde), a natural polyphenol compound isolated from the roots of radixSalviae Miltiorrhizae, is associated with a wide variety of biological activities and has been widely used in medicine as an antioxidant,anti-aging, an antibacterial and anti-inflammatory agent[1]. |
体外研究 (In Vitro) | Protocatechualdehyde (PCA) (50, 100 μM, 24/48 hours ) treated MCF-7 cells significantly decrease cell growth by 11% and 20% in 24 hours and by 22% and 27% in 48 hours, respectively[2]. Protocatechualdehyde (50, 100 μM, 24 hours) treated MCF-7 cells are increased by 1.9-fold and 2.6-fold in the concentrations of 50 μM and 100 μM, respectively. PCA suppresses proliferation of estrogen receptor (ER)-positive (MCF-7) breast cancer cells, but not ER-negative (MDA-MB-231) breast cancer cells[2]. Protocatechualdehyde (0, 100, 200 μM, 48 hours in HCT116 and SW480 cells) affects the enzyme activity of HDAC and observed that PCA treatment resulted in inhibition of HDAC activity in dose-dependent manner[3].
Cell Proliferation Assay[2] Cell Line: | Human breast cancer cell (MCF-7 and MDA-MB-231) | Concentration: | 0, 5, 10, 25, 50, and 100 μM | Incubation Time: | 24, 48 hours | Result: | Inhibited MCF-7 cells cell growth. |
Apoptosis Analysis[2] Cell Line: | Human breast cancer cell (MCF-7 and MDA-MB-231) | Concentration: | 0, 5, 10, 25, 50, and 100 μM | Incubation Time: | 24, 48 hours | Result: | Increased apoptosis in MCF-7 cells. |
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来源 | - Plants
- Compositae
- Hemistepta lyrata
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运输条件 | Room temperature in continental US; may vary elsewhere. |
储存方式 | 4°C, stored under nitrogen *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen) |
溶解性数据 | In Vitro: DMSO : ≥ 50 mg/mL(362.00 mM) *"≥" means soluble, but saturation unknown. 配制储备液 1 mM | 7.2401 mL | 36.2004 mL | 72.4008 mL | 5 mM | 1.4480 mL | 7.2401 mL | 14.4802 mL | 10 mM | 0.7240 mL | 3.6200 mL | 7.2401 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (stored under nitrogen)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用;
以下溶剂前显示的百 分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂: 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.5 mg/mL (18.10 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (18.10 mM,饱和度未知) 的澄清溶液。 以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。 2. 请依序添加每种溶剂: 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 2.5 mg/mL (18.10 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (18.10 mM,饱和度未知) 的澄清溶液。 以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。 3. 请依序添加每种溶剂: 10% DMSO 90%corn oil Solubility: ≥ 2.5 mg/mL (18.10 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (18.10 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。 *以上所有助溶剂都可在本网站选购。
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