Norisoboldine hydrochloride 是一种具有口服活性的天然芳基烃受体 (AhR) 激动剂。Norisoboldine 作为林参中主要的异喹啉类生物碱，可用于类风湿性关节炎和溃疡性结肠炎的研究。
| 生物活性 | Norisoboldine hydrochloride is an orally active naturalaryl hydrocarbon receptor(AhR) agonist. Norisoboldine hydrochloride, as a major isoquinoline alkaloid present in Radix Linderae, can be used for the research of Rheumatoid arthritis and Ulcerative colitis[1][2]. |
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体外研究
(In Vitro) | Norisoboldine hydrochloride (1~30 μM; 0~24 hours; CD4+T cells) activates AhR under hypoxic microenvironment and significantly downregulates mRNA expression of miR-31[2].
Norisoboldine hydrochloride (30 μM; 0~24 hours; CD4+T cells) inhibits glycolysis in hypoxia[2].
Norisoboldine hydrochloride (1~30 μM; 0~72 hours; Treg cells) promotes Treg differentiation in hypoxia[2].
Norisoboldine hydrochloride (10, 30 μM) facilitates the disassociation of HSP90/AhR complexes, the nuclear translocation of AhR, and the formation of AhR/ARNT complexes. Norisoboldine hydrochloride induces generation of Treg cells in hypoxia[2].
Western Blot Analysis[2] | Cell Line: | CD4+T cells | | Concentration: | 1~30 μM | | Incubation Time: | 24 hours | | Result: | Activated AhR in cells under hypoxic microenvironment. |
RT-PCR[2] | Cell Line: | CD4+T cells | | Concentration: | 1~30 μM | | Incubation Time: | 24 hours | | Result: | Significantly downregulated mRNA expression of miR-31. |
Immunofluorescence[2] | Cell Line: | CD4+T cells | | Concentration: | 30 μM | | Incubation Time: | 24 hours | | Result: | Inhibited glycolysis in hypoxia. |
Immunofluorescence[2] | Cell Line: | Treg cells | | Concentration: | 1~30 μM | | Incubation Time: | 72 hours | | Result: | Promoted Treg differentiation in hypoxia. |
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体内研究
(In Vivo) | Norisoboldine hydrochloride (10~40 mg/kg; p.o.; 20 days) significantly reduces the severity of joint swelling and erythema during the course of the experiment[1].
Norisoboldine hydrochloride (40 mg/kg; i.g.; 10 days) induces enhancement of CYP1A1 expression and suppresses expressions of Glut1 and HK2 in colons[2].
| Animal Model: | Male ICR mice (18–22 g) | | Dosage: | 10~40 mg/kg | | Administration: | P.o. | | Result: | Significantly reduced the severity of joint swelling and erythema during the course of the experiment. |
| Animal Model: | Female C57BL/6 mice (18–22 g) | | Dosage: | 40 mg/kg | | Administration: | I.g. | | Result: | Induced enhancement of CYP1A1 expression and suppressed expressions of Glut1 and HK2 in colons. |
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| 中文名称 | 去甲异波尔定（盐酸盐）； 去甲基异波尔定（盐酸盐）； 新木姜子碱（盐酸盐）； 去甲依波而定（盐酸盐） |
| 结构分类 | - Alkaloids
- Isoquinoline Alkaloids
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| 来源 | - Plants
- Lauraceae
- Lindera aggregata(Sims) Kosterm.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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