Nepetin (6-Methoxyluteolin) 是从Eupatorium ballotaefoliumHBK 分离的天然类黄酮，具有有效的抗炎活性。Nepetin 在 ARPE-19 细胞中抑制IL-6，IL-8和MCP-1分泌，IC50值分别为 4.43 μM、3.42 μM 和 4.17 μM。
| 生物活性 | Nepetin (6-Methoxyluteolin) is a natural flavonoid isolated fromEupatorium ballotaefoliumHBK with potent anti-inflammatory activities. Nepetin inhibitsIL-6,IL-8andMCP-1secretion withIC50values of 4.43 μM, 3.42 μM and 4.17 μM, respectively in ARPE-19 cells[1][2]. |
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体外研究
(In Vitro) | Pretreatment with Nepetin dose-dependently inhibited IL-6, IL-8 and MCP-1 secretion withIC50values of 4.43, 3.42 and 4.17 μM, respectively[1].
Nepetin (2.5-10 μM; 25 hours; ARPE-19 cells) treatment suppresses IL-1β-induced cytokine (IL-6, IL-8 and MCP-1) expression at mRNA level in ARPE-19 cells[1].
Nepetin (2.5-10 μM; 1.5 hours; ARPE-19 cells) treatment dose-dependently inhibits phosphorylation of IKKα/β and IκBα, and nuclear translocation of p65. Nepetin decreases the level of phosphorylated ERK1/2, JNK and p38 MAPK in activated ARPE-19 cells[1].
RT-PCR[1] | Cell Line: | ARPE-19 cells | | Concentration: | 2.5 μM, 5 μM, 10 μM | | Incubation Time: | 25 hours | | Result: | Suppressed the mRNA expression of IL-6, IL-8 and MCP-1 in ARPE-19 cells. |
Western Blot Analysis[1] | Cell Line: | ARPE-19 cells | | Concentration: | 2.5 μM, 5 μM, 10 μM | | Incubation Time: | 1.5 hours | | Result: | Dose-dependently inhibited phosphorylation of IKKα/β and IκBα, and nuclear translocation of p65. Decreased the level of phosphorylated ERK1/2, JNK and p38 MAPK in activated ARPE-19 cells. |
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| 来源 | - Plants
- Compositae
- Eupatorium ballotaefolium
- Plants
- Compositae
- Stevia rebaudiana(Bertoni) Bertoni
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
| 溶解性数据 | In Vitro: DMSO : 125 mg/mL(395.24 mM;ultrasonic and warming and heat to 60℃) 配制储备液 | 1 mM | 3.1620 mL | 15.8098 mL | 31.6196 mL | | 5 mM | 0.6324 mL | 3.1620 mL | 6.3239 mL | | 10 mM | 0.3162 mL | 1.5810 mL | 3.1620 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 2.08 mg/mL (6.58 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (6.58 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
*以上所有助溶剂都可在本网站选购。 |
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