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Jervine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Jervine图片
CAS NO:469-59-0
包装与价格:
包装价格(元)
20 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
蒜黎芦碱
11-Ketocyclopamine
Jerwiny
Iervin
产品介绍
Jervine 是一种有效的刺猬 (Hh) 抑制剂(IC50:500-700 nM)。它是一种天然致畸性甾体生物碱,来自于 Veratrumalbum 的根茎,具有抗炎和抗氧化作用。

产品描述

The biological activity of Jervine is mediated via its interaction with the 7 passes transmembrane protein Smoothened. Jervine binds with and inhibits smoothened, which is an integral part of the Hedgehog signaling pathways. With smoothened inhibited, the GLI1 transcription cannot be activated and Hedgehog target genes cannot be transcribed.

体外活性

Compared to controls, chondrocyte cultures exposed to 25 μg/ml jervine shows an apparent reduction in cell number but no differences in cellular morphology or relative intensity of alcian blue staining[2].

体内活性

Embryos exposed to jervine develop craniofacial abnormalities related to those found in Shh mutant embryos. The lower jaw of these embryos is severely reduced in size, often leading to loss of the incisors. Treatment of pregnant mice with the hedgehog inhibitor jervine induces jaw defects in the embryos similar to those found in Prx1?/?Prx2?/? embryos. The distal part of the jaw is reduced in size, and both incisors are absent[1].

细胞实验

Cells are grown in Eagle's basal medium supplemented with 10% fetal bovine serum and 0.25 mg/ml gentamicin sulfate. Cells are seeded onto 35-mm tissue culture plates at a density of 1×104 celldplate. The plated cells are incubated for 24 hours prior to treatment in a humidified incubator at 37 "C in an atmosphere of 5% CO2 in air. Drugs are then added: jervine to a final concentration of 5, 10, or 25 μg/ml; retinoic acid at 0.03, 0.30, or 3.0 pg/ml. After a final 48 hours of incubation, media are removed, the cultures are rinsed three times with phosphate-buffered saline (PBS) pH 7.4, and the cells are harvested into 0.5 ml of 0.1% (w/v) trypsin which contained 0.1% EDTA (w/v) in PBS. Each sample is mixed with 9.5 ml Isoton Ⅱ, and cell numbers are determined by using a Coulter Counter. (Only for Reference)

Cas No.

469-59-0

分子式

C27H39NO3

分子量

425.613

别名

蒜黎芦碱;11-Ketocyclopamine;Jerwiny;Iervin

储存和溶解度

Chloroform:Soluble
H2O:<1 mgml
DMSO:5 mg/mL (11.75 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years