Baicalein是一种黄嘌呤氧化酶抑制剂，其IC50值为 3.12 μM。

Baicalein is a xanthine oxidase inhibitor.

Baicalein suppresses mitogen-induced T cell proliferation and cytokine secretion in vitro. Pre-treatment with baicalein significantly suppresses Con A or anti-CD3/CD28 mAb-induced proliferation and cytokine secretion at 25 μM. Baicalein treatment induces DNA binding of NF-κB while inhibiting thioredoxin activity in the nuclear compartment[2]. Baicalein suppresses the proliferation, migration, and invasion of MDA-MB-231 cells in a time- and dose-dependent manner. Baicalein significantly decreases the expression of SATB1 in MDA-MB-231 cells. Baicalein also downregulates the expression of Wnt1 and β-catenin proteins and transcription level of Wnt/β-catenin-targeted genes[3].

Baicalein suppresses induction of graft versus host disease while not inhibiting the homeostatic proliferation of T-cells in mice. This observation clearly shows potent anti-inflammatory activity of baicalein in vivo[2]. Rats treated with baicalein are protected against an increase in heart to body weight ratio, plasma level of brain natriuretic peptides, intraventricular septum thickness, myocardial collagen volume of left ventricle (all P<0.05, respectively). The antifibrotic effects of baicalein are further illustrated by the suppressed expression of left ventricle pro-collagens I and III accompanied by the decreased expression of 12-lipoxygenase, and by reduced expression and activity of matrix metallopeptidase 9 and extracellular signal-regulated kinases. Baicalein can inhibit cardiac fibrosis in hypertensive rats[4].

MTT assay is conducted to evaluate the effect of baicalein on proliferation of breast cancer cells. MDA-MB-231 cells are routinely digested, collected, and then seeded in 96-well plates at a density of 8×103 cells/well. After incubation for 12-24 hours, cells are treated with 0, 20, 40, 60, 80, 100, and 120 μM baicalein according to their experimental grouping and then incubated at 37°C for 24, 48, and 72 hours[3].

491-67-8

C15H10O5

270.24

5,6,7-Trihydroxyflavone;黄芩素;黄芩黄素

DMSO:13.5 mg/mL (50 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years