Marein 能通过CaMKK/AMPK/GLUT1促进葡萄糖摄取,IRS/Akt/GSK-3β增加糖原合成,并通过Akt/FOXO1减少糖异生,从而改善 HepG2 细胞中高葡萄糖诱导的胰岛素抵抗。它对甲基乙二醛诱导的 PC12 细胞损伤具有神经保护作用,还有抗氧化、降压、降血脂和抗糖尿病作用。
产品描述
Marein shows neuroprotective effect on PC12 cell damage induced by methylglyoxal, which is due to a reduction of damage to mitochondria function and activation of the AMPK signal pathway, it may be a potent compound for preventing/counteracting diabetic encephalopathy.
体外活性
To clarify whether Marein, a major compound from the hypoglycemic plant Coreopsis tinctoria, prevents PC12 cell damage induced by MG, we cultured PC12 cells in the presence of MG and Marein. Marein attenuated MG-induced changes in the mitochondrial membrane potential (ΔΨm), mitochondrial permeability transition pores (mPTPs), intracellular Ca2+ levels, the production of reactive oxygen species (ROS), glutathione (GSH)/glutathione disulfide (GSSG) and adenosine triphosphate (ATP), and the increase in the percentage of apoptotic cells. Marein also increased glyoxalase I (Glo1) activity, phospho-AMPKα± (Thr172) and Bcl-2 expression and diminished the activation of Bax, caspase-3 and inhibitor of caspase-activated deoxyribonuclease (ICAD). Importantly, pretreatment of cells with Marein diminished the compound C-induced inactivation of p-AMPK. Molecular docking simulation showed that Marein interacted with the γ subunit of AMPK[1]
Cas No.
535-96-6
分子式
C21H22O11
分子量
450.39
别名
马里苷;马里甙
储存和溶解度
DMSO:60 mg/ml (133.21 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years