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ZCL278
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
ZCL278图片
CAS NO:587841-73-4
包装与价格:
包装价格(元)
5 mg电议
10 mg电议
50 mg电议
100 mg电议
200 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
ZCL 278
产品介绍
ZCL 278 是一种选择性 Cdc42 GTPase 抑制剂,与Cdc42结合,Kd为 11.4 μM。

产品描述

ZCL278 is a selective Cdc42 GTPase inhibitor.

体内活性

ZCL278 reduces the JUNV RNA load in the spleen more than 33-fold, with JUNV RNA being undetectable in 5 out of 8 mice. These results are similar to those seen in Gabapentin-treated mice, indicating that ZCL278 is able to abrogate JUNV replication[2].

激酶实验

Lyophilized Cdc42 protein is reconstituted to 5 mg/mL in a buffer consisting of 50 mM Tris, 0.5 mM MgCl2, 50 mM NaCl, 3% (wt/vol) sucrose, and 0.6% dextran. The stock solution is then diluted to 1 μM in 5 mM phosphate buffer, pH 7.4. Into a quartz cuvettete containing Cdc42 solution, aliquots of ZCL278 are added and incubated for 5 min before each fluorescent measurement. The excitation wavelength is 275 nm, and the fluorescence of tryptophan at 350 nm is measured after each addition. The titration curve is fitted using the equimolar specific binding model in GraphPad, and the Kd is calculated[1].

细胞实验

ZCL278 is prepared in DMSO and stored, and then diluted with appropriate medium before use[1]. To determine cell viability, PC-3 cells are incubated for 24 h with or without the Cdc42 activator, ZCL278, or NSC23766. By using the trypan blue dye exclusion method, the numbers of live and dead cells are obtained with a Countess Automated Cell Counter. P values are assigned in each experiment, and any null hypothesis with probability level<95% is rejected[1].

Cas No.

587841-73-4

分子式

C21H19BrClN5O4S2

分子量

584.89

别名

ZCL 278

储存和溶解度

DMSO:58.5 mg/mL (100 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years