Pachymic acid 是来自茯苓的一种三萜，可抑制Akt和ERK信号传导途径。

Pachymic acid is a natural product, and inhibits Akt and ERK signaling pathways.

Pachymic acid(PA) exhibited anti-tumor effects in vitro accompanied by induction of G2/M phase arrest and apoptosis in NCI-H23 and NCI-H460 lung cancer cells.?Mechanistically, our data showed that PA induced reactive oxygen species (ROS) production, resulting in the activation of both c-Jun N-terminal kinase (JNK) and endoplasmic reticulum (ER) stress apoptotic pathways in lung cancer cells.?Moreover, blockage of ROS production reversed PA-induced JNK and ER stress activation.?Finally, PA inhibited the growth of NCI-H23 xenograft tumors without causing any host toxicity, and inhibited cell proliferation and induction of apoptosis of tumor cells in tumor xenograft tissues[1].PA significantly reduced cell growth in a dose- and time-dependent fashion. Meanwhile, cell cycle arrest at G0 phase was induced by PA. PA also significantly inhibited cancer cell migration, invasion in a dose-dependent manner. Interestingly,demonstrated that cancer cell adhesion ability was suppressed dose-dependently, which may contribute to the inhibition of cell invasion. Finally, showed that PA inhibited AKT and ERK signaling pathways. And oncoproteins, such as PCNA, ICAM-1 and RhoA which are involved intumorigenesis, were also downregulated by PA[1].

The anti-proliferative ability of PA on lung cancer cells was assessed by MTT, colony formation and EdU proliferation assays.?Flow cytometric analysis was used to detect cell cycle changes.?Apoptosis was determined by annexin V/PI double-staining and the DNA ladder formation assays.?The expressions of the apoptosis-related proteins were analysed by western blot[1].

29070-92-6

C33H52O5

528.774

茯苓酸;3-O-Acetyltumulosic acid

DMSO:7 mg/mL (13.24 mM),Need ultrasonic
Powder: -20°C for 3 years
In solvent: -80°C for 2 years